PI3K抑制剂对肝星状细胞PI3K/AKT通路及Ⅲ型胶原表达的影响  

The influence of PI3K inhibitor on PI3K/AKT pathway in hepatic stellate cells and the expression of type Ⅲ collagen

作　　者：李娜[1] 肖倩[1] 祝聪聪[1] 王悦[1] 肖永红[1] 

机构地区：[1]河北联合大学公共卫生学院,河北唐山063000

出　　处：《现代预防医学》2014年第18期3377-3380,共4页Modern Preventive Medicine

基　　金：河北省自然科学基金资助项目(C2010001750)

摘　　要：目的探讨PI3K抑制剂(PI103)对肝星状细胞(HSC)PI3K/AKT通路及Ⅲ型胶原表达的影响,为肝纤维化的治疗提供理论基础。方法将液氮保存下的HSC株,于37℃,5%CO2孵育箱中复苏传代培养,同步化后将细胞分为3组:空白对照组、CCl4组、CCl4+PI3K抑制剂组。QPCR方法检测PI3K、AKT信号分子mRNA水平的变化;免疫细胞化学方法测定HSC的PI3K、AKT信号分子和Ⅲ型胶原表达情况。结果 CCl4组和空白对照组比较,PI3K、AKT信号分子mRNA水平和蛋白表达均增多,Ⅲ型胶原生成增多,差异有统计学意义(P<0.05)。CCl4组和CCl4+PI103组比较,PI3K、AKT信号分子mRNA水平和蛋白表达均减少,III型胶原生成减少,差异有统计学意义(P<0.05)。结论 PI3K抑制剂可降低PI3K、AKT信号分子及Ⅲ型胶原的表达,进而阻断肝纤维化进程。Objective The aim of this study was to discuss the effect of PI3K inhibitor （PI103） on PI3K/AKT pathway in hepatic stellate cells （HSC） and the expression of type Ⅲ collagen, in order to provide the theoretical basis for the treatment of liver fibrosis. Methods HSC line cells incubated in a 5% carbon dioxide incubation box at 37℃ were divided into three groups： blank control group, CCl4 group, CCL＋PI103 inhibitor group. QPCR method was used to detect the change in the mRNA level of PI3K and AKT in HSCs. The protein expression levels of PI3K, AKT and collagen Ⅲ were detected with immnnocytochemical method. Results Compared to the control group, the mRNA level and protein expression of PI3K and AKT, and the protein expression of type Ⅲ collagen as well, were all increased in CCl4 group, and the difference was statistically significant （P〈0.05）. Compared to CCl4 group, the expression of collagen type Ⅲ in CCl4＋PI103 group decreased, and the mRNA level and protein expression level of PI3K and AKT were also decreased, with statistically significant difference （P〈0.05）. Conclusion The PI3K inhibitor can reduce the expression of signal molecules PI3K and AKT and type Ⅲ collagen as well, indicating its potential to block the process of liver fibrosis.

关 键 词：PI3K/AKT通路 肝星状细胞(HSC) Ⅲ型胶原 

分 类 号：R319[医药卫生—基础医学]