富硒螺旋藻对日本血吸虫肝硬化小鼠肝组织端粒酶活性的影响  

Effect of Selenium-enriched Spirulina platensis on telomerase activity in mice cirrhosis tissue caused by Schistosoma japonicum

作　　者：李蜜[1] 徐标[1] 

机构地区：[1]华中科技大学同济医学院附属普爱医院,湖北武汉430032

出　　处：《现代预防医学》2014年第18期3387-3390,共4页Modern Preventive Medicine

摘　　要：目的探讨富硒螺旋藻抑制血吸虫肝硬化组织恶性变的作用及其机制。方法 80只小鼠感染日本血吸虫尾蚴后,随机均分为A、B、C、D4组,每组20只。A组(模型组)小鼠不作任何治疗。B组小鼠(吡喹酮组)在感染尾蚴6周时予吡喹酮500 mg/(kg·d)灌胃2 d,C组小鼠在感染尾蚴6周时予富硒螺旋藻100 mg/(kg·d)(富硒螺旋藻组)灌胃8周,D组小鼠在感染尾蚴6周时予吡喹酮500 mg/(kg·d)治疗2 d后再以富硒螺旋藻100 mg/(kg·d)(富硒螺旋藻+吡喹酮组)灌胃8周。另取10只小鼠作为正常组(E组)。第14周末分别留取各组小鼠肝组织,观察肝组织病理改变,测定其肝脏MDA含量、SOD活性以及端粒酶活性与端粒酶逆转录酶(TERT)表达变化。结果与A组比较,单纯吡喹酮或富硒螺旋藻治疗均可减轻肝纤维化程度,显著升高肝组织中SOD活性、降低MDA含量以及端粒酶活性与TERT表达水平(P<0.05)。与B组相比,富硒螺旋藻治疗后小鼠肝纤维化程度、SOD活性、MDA含量及端粒酶活性与TERT表达水平无明显变化(P>0.05)。与B组、C组相比,D组小鼠肝纤维化程度进一步减轻,肝组织中SOD活性显著升高、MDA含量以及端粒酶活性与TERT表达水平显著降低(P<0.05)。结论晚期血吸虫肝硬化组织因端粒酶活性增高具有恶变可能,富硒螺旋藻可通过降低肝组织氧化应激水平、抑制肝组织TERT表达及端粒酶活性而发挥抑制恶性变的作用。Objective This work was to study the inhibitory effect of selenium-enriched Spirulina on the malignant transformation of mice cirrhosis tissue caused by Schistosoma and the related mechanism. Methods Eighty mice infected with Schistosoma japonicum were equally divided into A, B, C and D group, with 20 mice/group. Group A was the control without any treatments. Six weeks after infection, group B was treated with praziquantel of 500 mg.kg-1.d-1 for 2 days; group C was treated with selenium-enriched Spirulina of 100 mg.kg-1.d-1 for 8 weeks; group D was treated with praziquantel of 500 mg.kg-1.d-1 for 2 days, then by selenium-enriched Spirulina of 100 mg.kg-1.d-1 for 8 weeks. Ten mice without Schistosoma infection nor any treatments were taken as normal control group （group E）. At the 14th week, all mice were sacrificed and a part of liver tissue were preserved to observe changes in hepatic histopathology. The contents of MDA and TERT, the activity of SOD and telomerase in liver tissue before and after treatments were measured. Results Compared to the group A, the therapy of praziquantet or selenium-enriched Spirulina alone could relieve the degree of hepatic fibrosis, reduce the contents of hepatic MDA and TERT, lower the activity of telomerase and greatly heighten the activity of SOD （P〈0.05）. No difference in the degree of hepatic fibrosis, the contents of MDA and TERT, the activity of SOD and telomerase was observed between group B and group C （P〉0.05）. Compared to the group C or group B, the degree of hepatic fibrosis, the contents of hepatic TERT and the activity of telomerase were obviously reduced and the activity of SOD was greatly increased （P〈0.05） in the group D. Conclusion The increase in telomerase activity in advanced liver cirrhosis tissue caused by schistosomiasis increased the potential of malignant transformation. Selenium-enriched Spirulina could inhibit the malignant transformation by inhibiting the expression of TERT and inhibit telomerase through reducing oxidative stress

关 键 词：血吸虫肝硬化 恶性变潜能 富硒螺旋藻 端粒酶逆转录酶 端粒酶 

分 类 号：R532.21[医药卫生—内科学]