湖南省成人外周血T淋巴细胞亚群的参考范围  

The establishment of reference ranges on peripheral blood T-lymphocyte subsets for healthy adults using flow cytometry in Hunan

作　　者：何志雄[1] 李木兰[2] 袁才佳[2] 蒋显勇[2] 

机构地区：[1]郴州市第一人民医院检验医学中心,湖南郴州423000 [2]湘南学院预防医学与医学检验系,湖南郴州423000

出　　处：《现代预防医学》2014年第18期3395-3397,共3页Modern Preventive Medicine

基　　金：湖南省精品课程项目(2009-252);湘南学院十二五重点学科项目(XNU125KD022)

摘　　要：目的建立湖南省健康成人外周静脉血T淋巴细胞亚群的参考范围。方法收集113名健康成人（18~60岁）静脉全血标本,用三色流式细胞术检测CD3＋、CD4＋及CD8＋淋巴细胞绝对数值,百分率和CD4＋/CD8＋比值。结果CD3＋、CD4＋和CD8＋淋巴细胞的绝对计数的中位数分别为1 422、736和546个/μl,CD4＋、CD8＋淋巴细胞的百分率的中位数为52.56%和39.98%,CD4＋/CD8＋比值的中位数为1.34。各参数值在不同年龄性别间分布差异无统计学意义（P〉0.05）。结论本研究成功建立了湖南省健康成人T淋巴细胞亚群正常参考范围,可为本地区临床医生提供有用的参考依据。Objective The purpose of this reasech was to establish the reference ranges on peripheral blood T-lymphocyte subsets in healthy adults in Hunan. Methods One hundred and thirteen whole blood samples were collected from healthy adult from 18 to 60 years old. Absolute counts and percentage of T-lymphocyte subsets of blood samples were calculated by three-color flow cytometer. Results The median values of the T-lymphocyte subsets were 1 422 cells/μl for CD3＋, 736 cells/μl for CD4＋ and 546 cells/μl for CD8＋. The median values of the percentage of T-lymphocyte subsets were 52.56% for CD4＋ and 39.98% for CD8＋, and the ratio was 1.34 for CD4＋/CD8＋. No significant differences were observed for different age and gender （P〉0.05）. Conclusion The reference ranges of peripheral blood T-lymphocyte subsets values were proposed by the current study for healthy adults in Hunan, providing more clinical information.

关 键 词：T淋巴细胞亚群 参考范围 流式细胞术 

分 类 号：R319[医药卫生—基础医学]