不同强度运动处方对青春期肥胖大鼠PPARγ及相关指标的影响  

Effects of different exercise intensity on PPARγ and relative index in adolescent obesity rats

作　　者：李萌[1] 柏友萍[1] 崔建飞[1] 许晓东[1] 戴园园[1] 张晶[1] 

机构地区：[1]安徽师范大学体育学院,芜湖241003

出　　处：《卫生研究》2014年第5期732-737,共6页Journal of Hygiene Research

基　　金：安徽省高校自然科学基金重点项目(No.KJ2013A138)

摘　　要：目的探讨低、中、高强度运动对饮食诱导青春期肥胖SD大鼠PPARγ及相关指标的影响。方法采用雄性SD大鼠建立肥胖大鼠模型,随机选取建模成功大鼠32只,分为对照组、低强度运动组、中强度运动组、高强度运动组,各运动组进行8周跑台训练。8周后采集大鼠血液及脂肪组织,测体重、体脂、血脂,用qRT-PCR法测脂肪组织PPARγmRNA的表达,免疫组化染色法测PPARγ蛋白表达,ELISA测血浆PPARγ浓度。结果运动后大鼠体重、体脂均降低(P<0.01),Lee’s指数组间无差异;TC表现为中、高强度运动组与对照组相比降低(P<0.05),TG水平低、中、高强度运动组均低于对照组(P<0.01),LDL水平中、高强度运动组高于对照组(P<0.05)。血浆PPARγ浓度低、中、高强度运动组比对照组高(P<0.05);脂肪组织PPARγmRNA的表达低、中、高强度运动组比对照组增强(P<0.05);PPARγ免疫组化结果与PPARγmRNA的表达情况一致。结论不同强度运动处方使青春期肥胖大鼠的体重、体脂明显降低,有效改善了血脂状况,并显著增加了血浆PPARγ的浓度和脂肪组织中PPARγmRNA的表达,使脂肪组织中PPARγ的反应增强,运动强度越大越明显。Objective Discuss the effects of low, medium, high intensity exercise induced by PPAR gamma and related indexes of diet in obese SD rats. Methods Male SD rats are selected to establish experimental obesity model of SD rats, selecting 32 rats of modeling successfully and randomly divided into control group , low intensity group , moderate intensity group and high strength group. Each exercise group is trained by treadmill training for 8 weeks. After 8 weeks, their blood and adipose tissue are collected, and the body fat and blood lipid are measured. The qRT-PCR method is used to measure the expression of PPARγ mRNA in adipose tissue, and immunohistochemical staining method are used to measure the protein expression of PPARγ, and the ELIASA is used to determine the plasma concentrations of PPARγ. Results After the exercise, theexponent of rats＇ weight and body fat have reduced （P 〈0.01 ） , and the exponent groups of lee＇ s index are indiserimination （ P 〉 0.05） , TC expressed as moderate intensity and high intensity decreased compared with the control group （P 〈 0.05）. TG levels in low, medium and high intensity group are lower than those in the control group （P 〈 0.01 ） , LDL levels in medium and high intensity groups are higher than the control group （P 〈 0.05）. The concentrations of PPAR plasma in low, medium and high intensity group are higher than in the control group （ P 〈 0.05 ）. PPARγ mRNA expression in adipose tissue of low, medium and high intensity group are enhanced than the control group. Immunohistochemical results of PPARγ is in consistent with the expression of PPARγ mRNA. Conclusion Method of different intensity of exercise for 8 weeks have play a significant effect on weight loss and body fat is significantly lower. Besides, it can also improve blood lipid effectively and significantly increases the expression of PPARγin plasma and adipose tissue.

关 键 词：运动强度 运动处方 青春期 肥胖 血脂 PPARΓ 

分 类 号：R179[医药卫生—妇幼卫生保健] G804.3[医药卫生—公共卫生与预防医学]