胃乃安新制剂对碘代乙酰胺致慢性浅表性胃炎的干预作用  

Effects of New Weinaian Preparation on Chronic Superficial Gastritis Induced by Iodoacetamide

作　　者：林传权[1] 陈娟[2] 李茹柳[1] 詹若挺[2] 苏碧茹 赖晓明 陈蔚文[1,2,4] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405 [2]广州中医药大学中药资源科学与工程研究中心,岭南中药资源教育部重点实验室,广州510006 [3]广州白云山中一药业有限公司,广州510530 [4]上海市高校中医内科学E-研究院,上海中医药大学,上海201203

出　　处：《中国实验方剂学杂志》2014年第19期146-150,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：粤港关键领域重点突破招标项目(GDDRC2007ZY002);上海市教育委员会E-研究院建设计划项目(E03008);中央财政支持地方高校发展专项资金项目(财教[2013]338号)

摘　　要：目的:观察胃乃安新制剂对慢性浅表性胃炎的作用。方法:SD大鼠按随机数字表分为正常组(10只)、模型组、胃乃安新制剂高、中、低剂量(9.10,2.87,0.91 g·kg-1)组和胃乃安胶囊(9.10 g·kg-1)组(每组各15只),予模型组及各给药组大鼠自由饮用0.1%碘代乙酰胺3周构建慢性浅表性胃炎模型的同时,各给药组予相应药物干预,模型组和正常对照组予等量自来水灌胃;3周后取胃组织从肉眼观察、病理切片、透射电镜3个层面评价胃乃安新制剂抗碘代乙酰胺致胃黏膜损伤作用,并检测胃组织的丙二醛(MDA)、超氧化物歧化酶(SOD)水平以探讨其可能的作用机制。结果:正常组胃组织未见明显损伤,未见明显炎细胞浸润;与正常组比较,模型组胃组织肉眼可见明显充血水肿,甚至糜烂,镜下见大量炎细胞浸润(P<0.01);由肉眼、病理评分结果,胃乃安新制剂高剂量组、胃乃安胶囊组均有抗胃黏膜损伤作用(与模型组比较P<0.01),且新制剂抗溃疡效果呈剂量依赖性关系;透射电镜观察结果表明,新制剂可明显逆转主细胞、壁细胞的超微结构损伤性改变;新制剂可降低胃组织MDA含量,提升SOD活性,可明显降低胃黏膜的自由基水平(与模型组比较,P<0.01)。结论:胃乃安新制剂对碘代乙酰胺所致慢性浅表性胃炎有显著抗损伤作用,可能通过清除胃黏膜自由基水平的途径发挥作用。Objective ： To in rat chronic superfical gastritis （CSG） normal control group （NC, n = 10） vestigate effect and mechanism of new Weinaian preparation （NWP） on the induced by iodoacetamide, nethed： SD rats were randomly divided into , CSG group （n = 15） NWP （9.10, 2.87, 0.91 g ·kg^-1） groups （n =15, respectively） and Weinaian capsule group （n = 15 ）. The CSG rats were induced by 0. 1% iodoacetamide for 3 weeks, the drug groups were given by gavage once a day for 3 weeks with appropriate drugs simultaneously, and the nomal contral and CSG groups were given the same amount of water. The effects of NWP on the CSG rats were evaluated by naked eye, pathological section and transmission electron microscope. The action mechanism of Weinaian was studied by detection the level of malondialdehyde （MDA） and superoxide dismutase （SOD） in the rat gastric mucosal. Result： Contrasted with the NC group, there was obvious injury and inflammation cell in the gastric mucosa of the CSG group. The effects of anti-injury were found in the ranitidine and high-dose group of Weinaian groups （P 〈 0.01 ） , there was dose-reponse relationship in the three Weinaian groups. The ultrastructure injury of chief and parietal cell were improved by the high-dose group of Weinaian. The group also reduced the level of MDA and increased the activity of SOD in the gastric mucosa （P 〈 0.01, vs CSG group）. Conclusion： The new Weinaian preparation can alleviate the gastric mucosal injury of CSG rat induced by iodoacetamide which maybe related to clearing gasrica mucosa radical.

关 键 词：胃乃安 碘代乙酰胺 慢性浅表性胃炎 自由基 

分 类 号：R285.5[医药卫生—中药学]