石榴鞣花多酚对糖尿病小鼠胰岛功能的影响  

Effects of Pomegranate Ellagic Polyphenol on Pacreatic Islet Function of Diabetes Mice

作　　者：张红[1] 马磊[2] 刘超翠 张曼[2] 张之 

机构地区：[1]新疆医科大学附属中医院药学部,乌鲁木齐830011 [2]新疆医科大学基础医学院药理教研室,乌鲁木齐830011

出　　处：《中国实验方剂学杂志》2014年第19期174-177,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区自然科学基金项目(2012211A038)

摘　　要：目的:观察石榴鞣花多酚对糖尿病小鼠胰岛功能的影响,初步探讨其作用机制。方法:采用腹腔注射链脲佐菌素制备糖尿病小鼠模型,造模成功小鼠随机分为糖尿病模型组,石榴鞣花多酚低、高剂量组,格列本脲组;另取正常小鼠做空白对照。灌胃给药30 d,测量体重和空腹血糖,进行糖耐量实验,取胰脏制备HE和免疫组化切片,观察胰脏病理组织学变化及胰岛素转录因子胰腺十二指肠同源盒-1(PDX-1)的表达。结果:与糖尿病模型组比较,石榴鞣花多酚组小鼠体重增加,糖耐量改善,空腹血糖降低明显,胰岛面积增加,PDX-1蛋白表达增加(P<0.05)。结论:石榴鞣花多酚具有一定改善糖尿病小鼠胰岛功能的作用,其作用机制可能与上调胰脏PDX-1的表达有关。Objective： The aim of this study was to observe the effects of pomegranate ellagic polyphenol （PEP） on paereatic islet function of diabetes mice. Method： Streptozotocin was intraperitoneally injected to induce diabetes in mice, and the diabetes mice were randomly divided into model group, low-dose PEP group, high-dose PEP group and glihenclamide group, while normal mice were taken as control group. PEP was intragastricly administrated for 30 d, body weight, fasting blood glucose, sugar tolerance were tested, HE and immunohistoehemistry pancreas slice were made to observe pathohistological changes and expression of transcription factor pancreatic duodenal homeobox-1 （PDX-1） . Result： Compared with diabetes mice, in mice with PEP administration, the body weight, pacreatic islet area and PDX-1 expression increased, while the fasting blood glucose decreased （P 〈 0.05）. Conclusion： PEP has a certain degree of effect on the improvement of pacreatic islet function in diabetes mice, and the machanism might be involved in upregulating the expression of protein PDX-1.

关 键 词：石榴鞣花多酚 糖尿病 胰岛功能 胰腺十二指肠同源盒-1 

分 类 号：R285.5[医药卫生—中药学]