海洋来源放线菌Actinoalloteichus cyanogriseus WH1-2216-6产浅蓝霉素A的发酵条件优化  

Fermentation optimization for the production of caerulomycin A from marine-derived Actinoalloteichus cyanogriseus WH1-2216-6

作　　者：贾海健[1] 王聪[1] 王乂[1] 刘培培[1] 朱伟明[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2014年第5期9-15,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金课题(21172204);国家重点基础研究发展计划课题(2010CB833804);国家高技术研究发展计划课题(2013AA092901);公益性行业科研专项(201405038))资助

摘　　要：目的对海洋来源放线菌Actinoalloteichus cyanogriseus WH1-2216-6产浅蓝霉素A的发酵条件进行优化,提高浅蓝霉素A的产量。方法对培养基的组成、接种量、起始pH、盐度、发酵时间、前体浓度及大孔吸附树脂添加量等条件的研究,通过单因素和正交试验,选择最优发酵条件;结果获得了浅蓝霉素A的最佳发酵条件,培养基组成(可溶性淀粉20g,甘油20g,蛋白胨20g,CaCO32g,2-吡啶甲酸400mg,XAD-16大孔吸附树脂50g,陈海水1L)、装液量150/500mL(体积分数)、5d种龄、3.3%盐度、起始pH=7.5、摇床(180r·min-1,28℃)发酵12d。结论以最佳发酵条件发酵,优化后的浅蓝霉素A的产量为优化前的7.0倍,达到610.5mg/L。Objective To improve the production of caerulomycin A from the marine-derived Actinoalloteichus cyanogriseus WH1-2216-6by the optimization of fermentation conditions.Methods The optimal composition of the medium,inoculum volume,pH,salinity,cultivation time,precursor concentration and addition of resin（XAD-16）were investigated by single-factor experiment and the orthogonal matrix methods.Results The optimal conditions were obtained as following：medium consisted of soluble starch 20 g,glycerol 20 g,peptone 20 g,CaCO32g,picolinic acid 400 mg,XAD-16 macroporous adsorptive resins 50 g,and sea water 1000mL;500mL conical flask containing 150 mL of culture medium;5dold of inoculum age;3.3% of salinity;pH 7.5;and 180 rpm at 28℃for 12 d.Conclusion The highest yield of caerulomycin A,i.e.610.5mg/L,obtained by A.cyanogriseus WH1-2216-6under the optimal fermentation conditions was 7.0-fold high as that in the original condition.

关 键 词：浅蓝霉素A 发酵优化 海洋放线菌 Actinoalloteichus cyanogriseus 

分 类 号：R931[医药卫生—生药学]