丹酚酸B抗小鼠日本血吸虫病肝纤维化的实验研究  

Study on salvianolic acid B against Schistosomiasis japonicum with hepatic fibrosis in mice

作　　者：郭鄂平[1] 杨树国[1] 张珍[2] 朱明磊[1] 王娅[1] 

机构地区：[1]湖北医药学院寄生虫学教研室,湖北十堰442000 [2]湖北医药学院附属太和医院检验部,湖北十堰442000

出　　处：《现代预防医学》2014年第19期3562-3565,3620,共5页Modern Preventive Medicine

基　　金：湖北省卫生厅专项科研项目(XF2010-26)

摘　　要：目的探讨丹酚酸B对日本血吸虫感染所致小鼠肝纤维化的保护作用及丹酚酸B抗纤维化的可能机制。方法随机将未感染和感染日本血吸虫尾蚴昆明小鼠分成A^F6组。即空白对照组(A)、模型组(B)、溶剂组(C)、丹酚酸B治疗低剂量[5 mg/(kg·d)]组(D)、中剂量[10 mg/(kg·d)]组(E)、高剂量[15 mg/(kg·d)]组(F),每组10只。实验结束处死小鼠,留取肝脏组织和血清,HE和VG胶原纤维染色观察小鼠肝组织变性、虫卵肉芽肿与胶原沉积等病理改变。同时测定血清和肝匀浆中丙二醛(Malondiadehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)、还原性谷胱甘肽(Glutathione peroxiase,GSH-px)及肝脏中羟脯氨酸(Hydroxyproline,Hyp)的含量。结果与A组相比,B、C、D、E、F组小鼠肝脏有明显虫卵肉芽肿形成、胶原沉积与肝纤维化,伴有不同程度肝细胞炎性损伤坏死。血清中MDA含量显著升高,SOD、GSH-px的含量显著降低,肝组织中MDA、SOD、GSH-px的含量变化与血清中相适应,肝组织内Hyp的含量也明显增加。给予丹酚酸B治疗后,D、E、F组均可以使小鼠肝组织坏死与胶原沉积明显减轻,虫卵肉芽肿面积减小,同时降低肝组织脂质过氧化产物MDA含量,提高SOD活性,增加GSH-px含量,与模型组比较差异均有统计学意义(P<0.01),同时肝脏Hyp的含量也降低,肝脏纤维化的程度减轻。结论丹酚酸B具有抗血吸虫性肝纤维化作用,可能与其增加SOD,GSH-px的含量,降低MDA的含量有关。Objective To study the effects and mechanism of salvianolic acid B（SAB） on hepatic fibrosis in mice induced by Schistosoma japonicum.Methods The mice model of hepatic fibrosis which infected by cercariae of S.japonicum were randomly divided into 6 groups：Group A as a blank control,Group B as a model,Group C as a solvent,Group D,E,F treated with a low dose of 5mg/（kg·d）,middle dose of 10mg/（kg·d）,and high dose of 15mg/（kg·d） of SAB,respectively.Each group had 10 mice.All the mice were killed at the end of the administration.Serum and liver tissues were kept back.Areas of egg granuloma and degrees of hepatic fibrosis were observed by HE and VG staining.The indexes about hepatic fibrosis were detected through MDA,SOD,GSH-px,Hyp.Results Compared with the blank control Group A,the egg granuloma appeared obviously,the collagen deposit and fibrosis occurred in liver tissues of Group B,C,D,E,F.The levels of MDA in sera and Hyp in liver tissues were significantly higher（P〈0.01）.However,the levels of SOD and GSH-px in sera were significantly lower（P〈0.01）.The levels of MDA and Hyp in the low,middle and high dose groups of SAB were significantly lower than those in the model Group B（P0.01）,the areas of egg granuloma,the collagen deposits and the degrees of hepatic fibrosis in Group D,E,F were significantly lower than those in the model Group B.Conclusion SAB has an obviously inhibitory effect on the hepatic fibrosis induced by Schistosoma japonicum infection.One of the possible mechanisms is to raise the activity of SOD,GSH-px and reduce the content of MDA in liver and serum.

关 键 词：丹酚酸B 日本血吸虫病 肝纤维化 

分 类 号：R115[医药卫生—公共卫生与预防医学]