丁型肝炎病毒抗原酶联免疫诊断试剂盒(HDAG-E)的制备和应用  

Preparation and Application of ELISA Kit(HDAG-E)for Detection of Serum Hepatitis D Antigen

作　　者：黄德庄 王全颖 贺丽香 李蜀南 闵福援 魏晓清 钟学顺 庄辉[2] 

机构地区：[1]北京第二传染病医院肝炎研究所,100054 [2]北京医科大学公共卫生学院

出　　处：《中国公共卫生学报》1991年第1期50-52,共3页

摘　　要：为了建立丁型肝炎病毒抗原酶联免疫诊断试剂,选用慢性肝炎患者的高效价抗-HD阳性血清用亲和层析法进行纯化;选用吐温20为裂解液处理血清,以裸露HDV核心部位的HDAg。本试剂采用酶联夹心法。经与澳大利亚及荷兰HEPAnoST1KA试剂盒HDAg对比,结果完全一致。与甲、乙型肝炎病毒抗原无交叉反应。用抗-HD阳性血清作中和试验确认其特异性可靠。对本院各类HBsAg阳性的肝炎患者或携带者血清测定结果,其HDAg的检出率为2.29％(9/392);国内8省、市同类病例HDAg检出率为1.37％(10/729)。临床及流行病学调查表明,本试剂可靠、实用。In order to develop the ELISA kit for the detection of HDV antigen(HDAg), we purified a high titer human anti-HD serum from a patient with chronic hepatitis D by affinity chromatography, and used Tween20 as a detergent treatment to release HDAg which occured in the core of HDV particles. Double antibodies sand with method was applicated.The results have been confirmed to be identical with standard HDAg from Australia and Holland. There was no cross reaction with hepatitis A or hepatitis B antingen. The results of neutranization test with anti-HD serum proved its specificity. Clinicial and epidemiological survey got a detective rate of 2.29 (9/392)and 1.37% (10/729) respectively. These reagents revealed an available and effective method.

关 键 词：丁型肝炎 HDAG-E 酵联夹心法 

分 类 号：R446.62[医药卫生—诊断学]