外源多胺对低温胁迫下黄瓜幼苗叶绿素荧光参数的影响  

Effect of Polyamines,Putrescine,Spermidine and Spermine on Chlorophyll Fluorescence Parameters of Cucumber Seedlings under Low Temperature Stress

作　　者：曹玉杰[1] 钱春桃[1] 薄凯亮[1] 程春燕[1] 陈劲枫[1] 

机构地区：[1]作物遗传与种质资源创新国家重点实验室.南京农业大学园艺学院,南京210095

出　　处：《中国瓜菜》2014年第5期10-13,共4页China Cucurbits And Vegetables

基　　金：国家重点基础研究发展计划(2009CB119001-01;2012CB113900);"863"计划专项(2010AA10A108;2012AA100202);江苏省科技支撑计划(BE2009310);江苏省农业科技支撑自主创新基金[CX(11)1002]

摘　　要：应用外源多胺(Polyamines,PAs)腐胺(Putrescine,Put)、亚精胺(Spermidine,Spd)、精胺(Spermine,Spm)处理2叶1心黄瓜幼苗,然后进行8℃/8℃低温及恢复处理,研究Put、Spd、Spm对黄瓜幼苗叶绿素荧光参数的影响。结果表明,在低温胁迫下黄瓜幼苗叶片叶绿素荧光参数最大荧光Fm、最大光化学效率Fv/Fm、PSII(光系统II)潜在活性Fv/F0均下降,初始荧光F0上升,表明PSII受到了伤害,使得PSII原初光能转换效率、PS潜在活性降低;与单纯低温胁迫相比,外源Put、Spd、Spm预处理均可以提高Fm、Fv/Fm、Fv/F0,而使F0降低。这一结果从叶绿素荧光动力学方面说明Put、Spd、Spm对改善黄瓜的耐冷性有一定的作用。Cucumber seedlings of two true leaves were sprayed by exogenous PAs （Put,Spd,Spm ） and then they were stressed under low temperature of 8℃/8℃for three days and recovered for one day. Effect of exogenous PAs on the changes of chlorophyll fluorescence parameters of cucumber seedlings under low temperature stress was studied. The results indicat-ed that the fluorescent parameters of chlorophyll, Fm,Fv/Fm and Fv/F0 appeared to decline and F0 appeared to increase, suggesting that PSII could be damaged,causing some decrease of primary light energy conversion and potential activities of PSII. Compared with the control treatment,exogenous PAs pre-treatment could significantly promote the increment of Fm, Fv/Fm,and Fv/F0,whereas decreased F0.The effect of exogenous PAs on cold-tolerance of cucumber is proved again by im-proving the changes of chlorophyll fluorescence parameters of cucumber seedlings.

关 键 词：黄瓜 低温胁迫 多胺 叶绿素荧光参数 

分 类 号：S642.2[农业科学—蔬菜学]