木薯转化酶抑制子MeINH3基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of Invertase Inhibitors Gene MeINH3 from Cassava(Manihot esculenta Crantz)

作　　者：吴晓慧[1,2] 耿梦婷[1,2] 范洁[1,2] 姚远[1,2] 符少萍[2] 胡新文[1] 郭建春[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2014年第9期1715-1720,共6页Chinese Journal of Tropical Crops

基　　金：国家973重大基础性项目(No.2010CB126600);国家自然科学基金(No.31170234);国家自然科学基金(No.31160061);木薯现代产业科技体系项目

摘　　要：转化酶抑制子调控转化酶的活性,在植物的糖代谢过程中具有重要作用。为了研究木薯的转化酶抑制子,本实验利用木薯基因组数据库分析及RT-PCR方法,从木薯中分离了1个木薯转化酶抑制子MeINH3的cDNA序列。MeINH3序列长度为564 bp,包含528 bp的完整开放阅读框,编码127个氨基酸,N端有16个氨基酸残基的信号肽,4个保守的半胱氨酸残基可形成两个二硫桥。亚细胞定位预测表明,MeINH3蛋白定位于胞外。根据生物信息学分析结果,推测其可能抑制木薯细胞壁转化酶活性。Invertase inhibitor controls the activity of invertase and plays a significant role in the sucrose metabolic processes in plants.An invertase inhibitor gene MeINH3 was isolated from cassava by cassava genome database analysis and RT-PCR.Analysis of the sequence indicated that the cDNA of MeINH3 was consisted of 564 bp with an open reading frame （ORF） of 528 bp encoding 127 amino acids.MeINH3 had a signal peptide including 16 amino acids at N terminus and four conserved cysteine residues forming two disulfide bridges.The subcellular localization prediction showed that MeINH3 protein was located extracellularly,suggesting that it may inhibit the activity of cassava cell wall invertase.

关 键 词：木薯 转化酶抑制子 基因克隆 生物信息学分析 

分 类 号：S533[农业科学—作物学] Q78[生物学—分子生物学]