木薯MeHDS2基因的克隆与分析  

MeHDS2 Gene Cloning and Analysis in Cassava

作　　者：王树昌[1,2] 于晓玲[1,2] 阮孟斌[2] 彭明[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2014年第9期1721-1726,共6页Chinese Journal of Tropical Crops

基　　金：国家重点基础研究发展973计划(No.2010CB126601);国家重点基础研究发展863计划(No.2012AA101204)

摘　　要：采用RT-PCR技术,从木薯SC124 cDNA中克隆得到一个具有完整阅读框的基因,命名为MeHDS2,该基因全长2 529 bp,编码842个氨基酸。蛋白质保守结构域分析结果表明,MeHDS2蛋白含有保守的1个HD结构域、1个b-zip结构域、1个START结构域和1个MEKHLA结构域;蛋白质空间结构预测显示其具有3个典型的α螺旋结构;亚细胞定位结果表明,MeHDS2蛋白在植物细胞核中特异表达,因此,推测其为HD-ZIP转录因子家族中的一员。基因表达分析结果表明,MeHDS2基因在木薯根、茎、叶中都有表达,但表达丰度不同。木薯干旱处理14 d后,自形态学顶端往下数第三、四片叶片的MeHDS2表达量显著高于根、茎及其他位置叶片组织。本研究为木薯抗旱分子育种提供了理论基础。Using RT-PCR technology,one gene sequence named MeHDS2 with 2 529 bp complete opening reading frame（ORF）was obtained from SC124,encoding a protein with 842 amino acids.MeHDS2 had a typical HD-domain,b-zip domain,START domain and a MEKHLA domain.Secondary structure of protein prediction showed that MeHDS2 had three typical α-spiral structures.Subcellular localization results showed that MeHDS2 was specifically expressed in nucleus of plant cells and it should be one member of the HD-Zip family.Gene expression analysis results showed that MeHDS2 was expressed differently in the testing samples,it was predominantly expressed in the 3rd-4th leaves after 14 days of drought treatment.We further speculated that MeHDS2 might have the function as a transcription factor to affect the development of cassava plants.The research provides a theoretical basis for drought resistance molecular breeding.

关 键 词：木薯 HD结构域 START结构域 表达载体 

分 类 号：S533[农业科学—作物学]