加味佛手散对原发性痛经模型大鼠血清雌二醇和孕酮浓度的影响  

Effects of additive Foshousan on the serum levels of estradiol and progesterone in rats with primary dysmenorrhea

作　　者：佟力 李兴岭 马帅 佟继铭 

机构地区：[1] 河北省青龙满族自治县人民医院妇产科, 秦皇岛066500 [2] 青龙满族自治县中医院内科 [3] 河北省中药研究与开发重点实验室中药药理毒理研究室, 承德067000

出　　处：《国际中医中药杂志》2014年第9期816-818,共3页International Journal of Traditional Chinese Medicine

基　　金：秦皇岛市科学技术研究与发展项目（项目编号：2012023A150）

摘　　要：目的：探讨加味佛手散（AFSS）对原发性痛经模型大鼠血清雌二醇（E2）和孕酮（P）浓度的影响。方法 SD大鼠60只，按体质量分层随机法分为正常对照组，模型组、AFSS低、中、高剂量组，元胡止痛片组，阳性对照组各10只。造模第6天起，在注射己烯雌酚的同时，AFSS低剂量组给予AFSS 0.75 g/kg、中剂量组给予AFSS 1.5 g/kg、高剂量组给予AFSS 3.0 g/kg，元胡止痛片组给予1.0 g/kg灌胃，给药容积为5 ml/kg；正常对照组、模型组同体积蒸馏水灌胃，连续7 d。采用ELISA法检测大鼠血清中 E2、P 水平，计算 E/P 比值。结果 AFSS 中、高剂量组血清中 E2含量[（48.27±6.42）pg/L、（47.51±7.03）pg/L]低于模型组（54.47±9.12）pg/L，P＜0.05；AFSS低剂量组E2含量（50.83±6.26）pg/L，与模型组比较差异无统计学意义（P＞0.05）；AFSS 各剂量组 P 含量分别为（687.41±21.14）ng/L、（720.47±41.03）ng/L、（719.78±32.01）ng/L，均高于模型组（667.32±46.51）ng/L（P＜0.05或0.01）；AFSS中、高剂量组P含量高于元胡止痛片组（699.31±36.31）ng/L（P＜0.05）。结论 AFSS中、高剂量组可降低E2、提高P含量、降低E2/P比值。Objective To investigate the effects of Additive Foshousan（AFSS） on the serum levels of estradiol and progesterone in rats with primary dysmenorrhea. Methods SD rats were divided into six groups, each 10. Group 1 was normal control , groups 2,3 and 4 were treated with low, middle and high dose of AFSS（0.75, 1.5 and 3.0 g/kg） respectively, group 5 as positive controls was treated with Yuanhuzhitong tablets （1 mg/kg）and group 6 served as model control. Except the normal control group, all rats were injected diethylstilbestrol and oxytocin to establish primary dysmenorrhea model. The levels of estradiol（E2）and progesterone（P） in rats serum was determined by ELISA method and the ratio of E2/P was calculated. Results In middle and high dose of AFSS groups, the level of E2（48.27±6.42）pg/L,（47.51±7.03）pg/L respectively were lower than that in model group（54.47±9.12）pg/L and the difference was statistically significant（P〈0.05）. In low dose of AFSS group, the level of E2 was（50.83±6.26）pg/L and the difference was no statistically significant compared with model group. In all doses of AFSS groups,The content of P（687.41±21.14）ng/L, （720.47±41.03）ng/L, （719.78±32.01）ng/L respectively were higher than that in the model control group （667.32±46.51）ng/L and the difference was statistically significant（P〈0.05 or〈0.01）. In middle and high dose of AFSS groups, the content of P were higher than that of Yuanhu-Zhitong tablets group（699.31±36.31）and the difference was statistically significant（P〈0.05）. Conclusion To reduce the content of E2, increase of P the content and decrease ratio of E2/P is one of the mechanism for AFSS to treat primary dysmenorrhea.

关 键 词：加味佛手散 原发性痛经 大鼠 雌二醇 孕酮 

分 类 号：R285[医药卫生—中药学]