鸡转录因子C/EBPα、KLF2、KLF3、KLF7、PPARα对L-FABP启动子活性的影响  

The Transcriptional Activity of L-FABP Promoter Regulated by Transcription Factors C/EBPα, KLF2,KLF3, KLF7, and PPARα in Chicken

作　　者：贺綦[1] 史洪岩[1] 王海霞[1] 高广亮[1,2] 王启贵[1,2] 李辉[1] 

机构地区：[1]东北农业大学动物科学技术学院,农业部鸡遗传育种重点实验室,黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,黑龙江哈尔滨150030 [2]重庆市畜牧科学院,重庆402460

出　　处：《中国畜牧杂志》2014年第17期13-17,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金资助项目(30972087);博士后研究人员落户黑龙江科研启动资助金(LBH-Q08131);"十一五"国家科技支撑计划项目(2008BADB2B01-7)

摘　　要：L-FABP是脂肪酸结合蛋白家族重要的成员。研究发现L-FABP在不饱和脂肪酸、饱和脂肪酸、胆固醇、胆汁酸等转运过程中扮演重要角色。目前,对哺乳动物L-FABP的活性调控机制的研究已经取得了一定进展,但在禽类上的相关报道还比较少见。为探讨鸡L-FABP基因的表达调控机制,本研究利用报告基因方法在人肝癌细胞系(HEGP2)中研究C/EBPα、KLF2、KLF3、KLF7、PPARα基因对鸡L-FABP基因启动子活性的影响。结果表明:C/EBPα显著抑制了鸡L-FABP基因的表达,KLF2、KLF3、KLF7、PPARα基因显著促进了L-FABP基因的表达,这些结果为深入研究鸡L-FABP基因表达调控机制奠定了基础。As an important member of the fatty acid binding protein （FABP） family,L-FABP plays important roles in the translocation of saturated fatty acid,unsaturated fatty acid,cholesterol,bile acid,ect.In mammals,certain progresses were made on the regulatory mechanism of L-FABP transcriptional activities.However,it is rarely reported in avian species.In order to investigate the regulatory mechanism of L-FABP gene transcription in chicken,we cotransfected C/EBPα,KLF2,KLF3,KLF7,PPARα and examined their individual effects on L-FABP promoter activity,using a routine Luciferase report gene method in the human hepatocellular carcinoma cell line,HEPG2.The expression of L-FABP was significantly repressed by C/EBPα,but significantly promoted by KLF2,KLF3,KLF7 and PPARα,respectively.These results will provide a foundation to stimulate further research on the regulatory mechanism and function of chicken L-FABP gene.

关 键 词：L-FABP基因 启动子 活性分析 鸡 

分 类 号：S831.2[农业科学—畜牧学]