HIV金标法检测在无偿献血中的应用效果分析  

Evaluation of HIV immune colloidal gold technique implemented in pre-donation screening in Xi'an,China

作　　者：王文[1] 张国权[1] 赵晓华[1] 曹晓丽[1] 

机构地区：[1]陕西省血液中心,陕西西安710061

出　　处：《中国输血杂志》2014年第8期858-860,共3页Chinese Journal of Blood Transfusion

摘　　要：目的评估HIV金标法检测在无偿献血中的应用效果。方法对使用HIV金标法进行献血者筛查前后1年,血液HIV ELISA检测和WB确认情况进行对比分析。结果快筛前(对照组)ELISA法检出抗-HIV阳性546人(0.32%)、双阳92人(0.0538%);快筛后(观察组)检出抗-HIV阳性531人(0.29%)、双阳17人(0.0094%)。观察组与对照组ELISA抗-HIV阳性率无明显下降(χ2=18.2,P>0.05),双阳人数显著下降(χ2=55.80,P<0.01);观察组和对照组WB阳性分别为5人(0.028‰)、81人(0.474‰),快筛后HIV感染者献血率(WB确认阳性率)显著降低(χ2=71.4,P<0.01)。结论在采血现场开展HIV金标法检测可以大大降低HIV阳性确认的比例,同时应加大对献血者的宣传和教育,引进先进技术,提高输血的安全性。Objective To evaluate the effect of HIV immune colloidal gold technique detection results among voluntary blood donors in Xi＇an. Methods For a year before and after using HIV colloidal gold strips for pre-donation screening, HIV ELISA detection and WB confirmation cases were employed for anti-HIV detection in after donation screening for blood sam- pies. The anti-HIV confirmatory test was performed by WB. Results The HIV reactive rate of ELISA did not show significant differences between observation group and control group （χ2 = 18. 2,P 〉 0.05 ）, however, the positive rate of anti-HIV in ini- tim and recheck testing showed a significant difference （χ2 = 55.80,P 〈 0. 01 ）. Also, HIV positive rate of WB showed a sig- nificant difference （χ2 = 71.4, P 〈 0.01 ）. Conclusion Based on current situation of HIV infections in blood donors in Xi＇ an,HIV colloidal gold strips were used for blood pre-donation screenin~ which will helo to reduce the high-risk donor for do-nation. Besides, the publicity and education of HIV knowledge,the implementation of more secured blood test, and the introduction of advanced technology to guarantee the blood safety should be performed.

关 键 词：无偿献血 抗-HIV检测 金标法 

分 类 号：R446.61[医药卫生—诊断学]