核酸适配体生物传感器对心肌肌钙蛋白Ⅰ的检测研究(英文)  

The Detection of cTnⅠ by The Aptamer Biosensor

作　　者：蒋树海[1] 范婷[1] 刘利娟[1] 陈怡[1] 张晓清[1] 沙作良[1] 刘玉良[1] 张娟琨[1] 

机构地区：[1]天津科技大学生物工程学院,天津市工业微生物重点实验室,教育部工业微生物重点实验室,天津300457

出　　处：《生物化学与生物物理进展》2014年第9期916-920,共5页Progress In Biochemistry and Biophysics

基　　金：supported by grants from the Ministry of Science and Technology of China(2013DFG52490);Tianjin Municipal Science and Technology Commission(12ZXCXSY07500);The National Natural Science Fundation of China(21076157)~~

摘　　要：核酸适配体生物传感器是利用固定在电极表面的适配子与被测溶液中心肌肌钙蛋白Ⅰ（cTnⅠ）发生特异性结合,从而达到检测的目的.我们对玻碳电极进行阳极氧化、氨基化修饰,通过碳二亚胺盐酸盐（carbodiimide hydrochloride,EDC）、N-羟基琥珀酰亚胺（N-hydroxysuccinimide,NHS）活化作用将适配子结合在电极表面.cTnⅠ最佳检测范围是0.05-5 nmol/L,最低检测限为0.05 nmol/L,检测时间为5 min.A new aptamer biosensor for detection of cTn I was established. It can detect cTn I in samples. The surface of GC was modified with anode-oxidation, amidation and activation modification. We used EDC and NHS as activators. Due to aptamer＇s specificity for cTn I, we can detect the concentration of cTn I in sample solution. The linear range of the analytical signal is observed from 0.05 -5 nmol/L. The lowest detected concentration is 0.05 nmol/L. The detection time is 5 min.

关 键 词：核酸适配体 生物传感器 cTnⅠ 

分 类 号：O65[理学—分析化学]