环丙沙星对回盲部切除术后白细胞介素-10基因敲除小鼠肠道炎性反应的影响  

Effects of ciprofloxacin on the enteritis of interleukin-10 knockout mice after ileocaecal resection

作　　者：吴茸[1] 郭振[1] 李毅[1] 顾立立[1] 龚剑峰[1] 朱维铭[1] 李宁[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院全军普通外科研究所,210002

出　　处：《中华消化杂志》2014年第9期607-610,共4页Chinese Journal of Digestion

基　　金：卫生行业专项基金资助（201002020）

摘　　要：目的研究环丙沙星对行回盲部切除术后 IL-10基因敲除小鼠（CD 模型小鼠）的吻合口及小肠炎性反应及纤维化的影响。方法将12只敲除 IL-10基因的雄性 C3H/HeJBir 小鼠平均分成对照组和环丙沙星组，小鼠行回盲部切除、回肠结肠吻合术建立模型后，对照组无任何干预措施，环丙沙星组予口服环丙沙星50 mg·kg^-1·d^-1，共4周。处死所有小鼠，取吻合口附近肠道组织行 HE 及天狼星红染色，并进行组织学评分，用 ELISA 法检测吻合口附近肠道组织及小肠 IFN-γ、TGF-β的含量，用实时定量 PCR 检测前胶原α1含量。采用 t 检验进行组间比较。结果环丙沙星治疗组小鼠吻合口（2．50±0．55）和小肠（1．67±0．52）炎性反应水平较对照组（3．67±0．63、2．83±0．75）明显下降，差异均有统计学意义（t＝-8．273、-5．188，P 均＜0．05）；并且 IFN-γ的水平也明显下降，分别为（0．72±0．22）pg/mg 比（1．76±0．43）pg/mg 和（0．23±0．12）pg/mg 比（0．78±0．52）pg/mg，差异均有统计学意义（t ＝-7．354、-5．268，P 均＜0．05）。但环丙沙星并不能明显降低小鼠的吻合口及小肠的纤维沉积，TGF-β的水平与对照组相比，差异无统计学意义（P ＞0．05）。结论环丙沙星减轻 CD 模型小鼠吻合口炎性反应的作用有限，术后可能需要维持用药，才能减少吻合口疾病复发。Objective To investigate the effects of ciprofloxacin on anastomotic stoma,small intestinal inflammation and fibrosis in interleukin-10 knock-out mice （mice model of Crohn′s disease （CD）） after ileocaecal resection.Methods Twelve interleukin-10 knockout male C3H/HeJBir mice were evenly divided into control group and ciprofloxacin group.The mice model was established with ileocaecal resection and ileocolostomy.There was no intervention in control group.Ciprofloxacin （50 mg/kg per day）was orally administrated in ciprofloxacin group for four weeks.All the mice were executed.The intestine around anastomotic stoma was taken for hematoxylin-eosin （HE）and Sirius red staining and histological scoring.The level of interferon （IFN）-γand tumor growth factor （TGF）-βin intestine around anastomotic stoma and small intestine was detected by enzyme-linked immunosorbent assay （ELISA） method.The content of procollagen α1 was determined by real-time polymerase chain reaction.The data were analyzed by t test.Results The inflammatory reaction level of anastomotic stoma and small intestine in mice of ciprofloxacin group （2.50±0.55 and 1 .67±0.52）was significantly lower than those of control group （3.67±0.63 and 2.83±0.75 ,t=-8.273,-5 .188,both P 〈0.05 ）.The IFN-γof anastomotic stoma and small intestine also significantly decreased （（0.72 ±0.22 ）pg/mg vs （1 .76 ±0.43 ）pg/mg, （0.23±0.12）pg/mg vs （0.78 ± 0.52 ）pg/mg;t = - 7.354,- 5 .268,both P 〈 0.05）.However, ciprofloxacin could not significantly decrease fibrosis in anastomotic stoma and small intestine of CD mice. There was no significant difference in the level of TGF-βcompared with control group.Conclusions The role of ciprofloxacin in improving inflammatory reation in anastomotic stoma of CD mice was limited. Maintenance therapy may be necessary to reduce the relapse of anastomotic stoma diseases.

关 键 词：环丙沙星 炎性肠疾病 小鼠 

分 类 号：R656[医药卫生—外科学]