脐血单个核细胞的分离及冻存  

Separation and Cryopreservation of Cord Blood Mononuclear Cells

作　　者：兰炯采[1] 刘忠[1] 甘茂州[2] 陈强[3] 张印则 孟庆宝[1] 

机构地区：[1]第一军医大学南方医院输血科,广州510515 [2]四川省人民医院,成都610041 [3]中国医学科学院输血研究所,成都610081 [4]张家口中心血站,张家口075000

出　　处：《中国实验血液学杂志》2002年第4期351-354,共4页Journal of Experimental Hematology

基　　金：四川省卫生厅"九五"重大项目;四川省科委重点项目资金资助项目 编号川卫科教(97-040)970105

摘　　要：研究脐血采集后的放置、单个核细胞的分离、冻存等的影响因素。方法为血液采集后,离心分离单个核细胞,进行冷冻保存及复苏后洗涤。结果优选为采集后4℃或室温放置24小时内,羟乙基淀粉2次离心法分离单个核细胞,50％二甲亚砜加自身血浆为保护剂冻存、复苏后洗涤等程序。结论:本研究的优化方法可有效地保存脐血单个核细胞。The influencing factors on cord blood storage after collection and mononuclear cell separation as well as cryopreservation were studied.The mononuclear cell are separated from blood after blood collection,then cryopreserved and washed after thawed.Results showed that the cord blood kept at 4C or room temperature less than 24 hours after blood collection,mononuclear cell separated by hydroxyethylstarch and 2 centrifugations,mononuclear cell cryopreserved with 50% DMSO and autoplasma from cord blood as protectives and washing the cells after thawing.In conclusion,the optimal project in this study can effectively preserve cord blood mononuclear cells.

关 键 词：脐血 单个核细胞 细胞分离 冷冻保存 造血干/祖细胞移植 

分 类 号：R457.1[医药卫生—治疗学]