一种基于微流控芯片的胚胎动态培养方法  

A Dynamic Method of Embryo Culture Based on A Microfluidic Chip

作　　者：王维[1] 彭娅娅[1] 梁广铁[1] 廖早文 刘大渔[1] 

机构地区：[1]广州医科大学广州市第一人民医院检验科,广州510180

出　　处：《生殖与避孕》2014年第9期702-705,724,共5页Reproduction and Contraception

基　　金：国家自然科学基金;项目号:81271730

摘　　要：目的:建立一种接近生理状态的胚胎动态培养方法。方法:按功能设计和构建微流控芯片,使小鼠体外受精卵在微流控芯片微孔中接受持续的灌注培养,同时模拟输卵管收缩和纤毛运动引起的流体机械刺激和生化刺激,并与常规微滴培养法比较,监测胚胎发育情况和囊胚形成率。结果:微流控芯片动态培养方法可显著改善胚胎的质量。与常规微滴培养法相比,微流控动态培养获得的4-细胞胚胎、桑椹胚和囊胚形成率均有显著提高(68.4±1.2%vs 53.2±2.5%;55.3±2.6%vs 45.5±3.3%;40.5±2.7%vs 35.5±2.3%)(P<0.05)。而2种方法获得的卵裂率无统计学差异(75.5±3.2%vs 73.9±4.2%,P>0.05)。结论:在微流控芯片上实现了小鼠近生理状态的胚胎培养,显著改善了胚胎质量,有希望成为一种胚胎培养的有力工具。Objective： To develop a microfluidic chip for embryo dynamic culture method nearly physiological status. Methods： A microfluidic chip was designed and fabricated for testing the influence of continual fluid flow on embryo development. This gave rise to design of a microfluidics system using microchannels as conduits for fluid flow through a 16-microcellular where mouse embryos resided by mimicking the fluid- mechanical and biochemical stimulation embryos experience in vivo from ciliary currents and oviductal contractions. And compared with conventional droplet culture method, monitoring of the embryonic development conditions and blastocyst formation rate. Results： The microfluidic chip dynamic culture method can significantly improve the embryo development. The microfluidic dynamic method was superior to the microdrop-static method in terms of 4-cell embryo rate （68.4 ± 1.2% vs 53.2 ± 2.5%）, morula rate （55.3± 2.6% vs 45.5 ± 3.3%） and blastocyst rate （45.5 ± 2.7% vs 35.5± 2.3%）（P〈0.05）. These two methods didn＇t show significant difference in the rate of 2-cell embryos （75.5 ±3.2% vs 73.9 ±4.2%, P〉0.05）. Conclusion： Physiological embryo culture was achieved on a microfluidic chip. This microfluidic method was able to improve embryo development and showed advantage over the conventional method, which was expected to serve as a powerful tool for embryo culture in the future.

关 键 词：微流控芯片 动态培养 胚胎发育 

分 类 号：Q813.7[生物学—生物工程] R714.8[医药卫生—妇产科学]