姜黄素不能抑制无镁人工脑脊液诱发大鼠海马切片产生的癫癎样放电  

Failure of Curcumin in inhibiting epileptiform discharge of hippocampal slices of rat induced by Mg^(2+) -free artificial cerebrospinal fluid

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作  者:常燕燕[1] 宫新伟[2] 龚海庆[2] 张溥明[2] 梁培基[2] 陆钦池[1] 

机构地区:[1]上海交通大学医学院附属仁济医院神经内科,上海200127 [2]上海交通大学生物医学工程学院,上海200240

出  处:《上海交通大学学报(医学版)》2014年第9期1332-1337,1342,共7页Journal of Shanghai Jiao tong University:Medical Science

基  金:上海交通大学"医工(理)交叉研究基金"(YG2012ZD08)~~

摘  要:目的通过与抗癫癎药物丙戊酸(VPA)的作用比较,探讨姜黄素对癫癎样放电活动有无直接抑制作用。方法利用多电极记录系统记录无镁人工脑脊液(ACSF)诱发大鼠海马切片产生的癫癎样放电。成功诱导出癫癎样放电后,加入100μmol/L姜黄素或3 mmol/L丙戊酸,观察其对癫癎样放电的作用。结果无镁ACSF持续灌流15 min左右,多电极阵列上可记录到海马各区域的癫癎样放电。100μmol/L姜黄素对癫癎样放电的放电频率、持续时间、传播速度等均无明显影响(P>0.05)。3 mmol/L VPA能使癫癎样放电的放电频率降低(P<0.05),逆行方向的传播速度降低(P<0.05)。结论 100μmol/L姜黄素对无镁ACSF诱发的大鼠海马切片的癫癎样放电无抑制作用。Objective To investigate whether curcumin has direct inhibitory effect on the epileptiform discharge by comparing the effects of curcumin and antiepileptic drug of valproate. Methods The epileptiform discharge of hippocampal slices of the rat induced by Mg2+-free artificial cerebrospinal fluid (ACSF) was recorded by the multi-electrode array (MEA). After epileptiform discharge was successfully induced, curcumin of 100μmol/L or valproate of 3 mmol/L was applied and their effects on the epileptiform discharge were observed. Results After Mg2+-free ACSF was continuous perfused for 15min, the epileptiform discharge of all regions of hippocampal slices was recorded by the MEA. Curcumin of 100 μmol/L did not significantly affect the frequency, duration, and propagation speed of epileptiform discharge ( P 〉 0.05). Valproate of 3 mmol/L significantly decreased the frequency of epileptiform discharge (P〈0.05) and the propagation speed at retrograde direction (P〈0.05). Conclusion Curcumin of 100 μmol/L fails to inhibit the epileptiform discharge of hippocampal slices of the rat induced by Mg2+ -free ACSF.

关 键 词:癫癎样放电 多电极阵列 姜黄素 丙戊酸 海马切片 

分 类 号:R285.5[医药卫生—中药学]

 

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