大鼠下丘脑腹外侧视前区和结节乳头体核的直接神经纤维投射研究  

The research of direct nerve fiber projections between ventrolateral preoptic nucleus and tuberomammillary nucleus in rats

作　　者：丁丁[1,2] 丁睿[1] 吴芳[1] 张瑾[1] 许奇[1] 解敏[1] 王烈成[1] 

机构地区：[1]安徽医科大学基础医学院生理学教研室,合肥230032 [2]安徽医学高等专科学校基础部生理学教研室,合肥230601

出　　处：《安徽医科大学学报》2014年第10期1376-1378,共3页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金资助项目(编号:81071075);教育部博士点基金新教师类(编号:20093420120004);安徽医科大学校科研基金(编号:2013XKJ002;2013XKJ005)

摘　　要：目的探讨促睡眠调节中枢下丘脑腹外侧视前区(VLPO)与促觉醒调节中枢结节乳头体核(TMN)之间是否具有双向调节的直接通路。方法将SD大鼠随机分为对照组(ACSF组)与实验组(TMN+ACSF&VLPO+DiO组,TMN+DiO&VLPO+ACSF组),采用脑立体定位技术,核团内微量注射、冰冻切片等方法观察和记录大鼠VLPO和TMN分别注射细胞膜荧光探针Fast DiO后的TMN和VLPO的荧光信号。结果 TMN+ACSF&VLPO+DiO组大鼠注射Fast DiO术后72 h,TMN脑区神经细胞可见明显的绿色荧光;TMN+DiO&VLPO+ACSF组大鼠注射Fast DiO术后72 h,VLPO脑区神经细胞也可见明显的绿色荧光。结论 VLPO与TMN间有双向直接神经纤维投射。Objective To investigate the research of the direct nerve fiber projections between ventrolateral preop-tic nucleus （ VLPO） and tuberomammillary nucleus （ TMN） in rats. Methods SD rats were randomly divided into control group （ ACSF group） and experimental groups （ TMN＋ACSF＆amp;VLPO＋DiO group and TMN＋DiO＆amp;VLPO＋ACSF group） . Brain stereotaxic technique, microinjection and frozen section technique were used to detect and record the fluorescence signal in TMN and VLPO after injecting fluorescence dye, Fast DiO, into VLPO and TMN, respectively. Results In TMN＋ACSF＆amp;VLPO＋DiO group, the green fluorescence signals in TMN could be de-tected after injected Fast DiO dye 72 h. Meanwhile, in TMN＋DiO＆amp;VLPO＋ACS group, the green fluorescence signals could be detected in VLPO after injected Fast DiO dye 72 h. Conclusion There have bi-directional nerve fiber projection between VLPO and TMN.

关 键 词：腹外侧视前区 结节乳头体核 大鼠 突触 

分 类 号：R338.63[医药卫生—人体生理学]