预测蛋白质可结晶性研究进展  

Progresses in predicting the crystallizability of proteins

作　　者：周仁斌[1] 卢慧甍[1] 尹大川[1] 

机构地区：[1]西北工业大学生命学院空间生物实验模拟技术国防重点学科实验室,陕西西安710072

出　　处：《生物工程学报》2014年第9期1362-1371,共10页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31170816);国家重点基础研究发展计划(973计划)(No.2011CB710905);西北工业大学基础研究基金(No.JC20110285)资助~~

摘　　要：解析蛋白质的三维结构具有重要的生物学意义,更是蛋白质功能研究和理性药物设计的基础。目前解析蛋白质结构最重要的方法是X-射线衍射晶体学解析技术。但是运用该技术解析蛋白质结构的关键是获得高质量的蛋白质晶体。然而,据统计仅有42%的可溶纯化蛋白质能够得到晶体,即不同蛋白质的可结晶性表现不同。由于实验方法验证蛋白质的可结晶性耗时耗力,因此,有研究者运用计算机模拟的方法预测蛋白质的可结晶性,从而节省资源与成本并且提高实验的成功率。本文结合我们的研究工作,介绍了几种目前较为成功的蛋白质可结晶性预测方法及其研究途径。Determination of protein 3-dimensional structure offers very important information in biology researches, especially for understanding protein functions and redundant drug design. The X-ray crystallography is still the main technique for protein structure determination. Obtaining protein crystals is an essential procedure after protein purification in this technique. However, there is only 42% of soluble purified proteins yield crystals by statistics. Experimental verification of protein crystallizability is relatively expensive and time-consuming. Thus it is desired to predict the protein crystallizability by a computational method before starting the experiment, In this paper, combined with our own efforts, some successful in silico methods to predict the protein crystallizability are reviewed.

关 键 词：蛋白质结构 X-射线衍射晶体学 蛋白质可结晶性预测 

分 类 号：Q51[生物学—生物化学]