先天性氯离子腹泻致病基因Slc26a3在雄性啮齿类动物生殖道中的表达定位  

Expression pattern of congenital chloride diarrhea pathogenic gene Sic26a3 in the reproductive tract of male rodents

作　　者：陈良榉 许文明[1] 

机构地区：[1]四川大学华西第二医院、四川大学香港中文大学生殖医学联合实验室、教育部出生缺陷与相关妇儿疾病重点实验室,成都610041

出　　处：《中华医学遗传学杂志》2014年第5期654-658,共5页Chinese Journal of Medical Genetics

基　　金：教育部“新世纪优秀人才支持计划”（NCET-12-0382）

摘　　要：目的通过检测小鼠和大鼠附睾、睾丸、精子中Slc26a3基因的表达模式，探索SLC26A3的表达模式是否与先天性氯离子腹泻（congenital chloride diarrhea，CLD）男性的生育力低下有关。方法应用免疫组织化学技术和Western印迹用于研究S1c26a3在大鼠、小鼠附睾上皮中的表达定位。应用Western印迹检测Slc26a3在大鼠睾丸发育过程中的表达模式，免疫荧光和Western印迹用于研究Sic26a3在小鼠精子上的定位。同时应用Western印迹在CFTR敲除小鼠以及CFTR定点突变小鼠的睾丸和附睾中检测Slc26a3的表达是否有改变。结果Slc26a3的表达强度在小鼠和大鼠附睾的头部、体部、尾部呈逐渐降低的趋势；在大鼠睾丸中随发育过程也呈渐弱的动态模式；Slc26a3在小鼠精子尾部的主段有表达；在CFTR基因敲除小鼠以及CFTR定点突变小鼠的睾丸和附睾中Slc26a3的表达没有显著变化。结论Slc26a3在啮齿类动物雄性生殖系统的表达模式与其功能密切相关，CLD男性患者生育力低下可能与SLC26A3在附睾的酸碱平衡调控中的重要作用有关。Objective To determine the expression pattern of Slc26a3 gene in reproductive tract of male rodents to clarify whether the expression pattern is related to the subfertility observed in congenital chloride diarrhea （CLD） disease. Methods The expression of Slc26a3 in mouse and rat epididymis has been studied with immunohistochemistry and Western blotting. Its developmental expression pattern in rat testis was detected by Western blotting, while both of immunofluorescence and Western blotting were used to localize the expression of Slc26a3 in mouse sperms. The potential change of Slc26a3 expression in CFTR （cystic fibrosis transmembrane conductance regulator） knockout mice and CFTR mutant mice was also detected with Western blotting. Results The expression level of Slc26a3 gradually decreased along epididymis from its caput to corpus, then to its cauda part. This gradually decreasing expression pattern was also found in rat testis during development. Slc26a3 was localized mainly on the trunk of mouse sperm tail. In the testis and epididymis of CFTR knockout mice and CFTR mutant mice, no significant change of Slc26a3 expression was found. Conclusion Slc26a3 is expressed in male reproductive tract, and its expression pattern is related to the function. Thus, the subfertility observed in CLD disease may be related to the important role of SLC26A3 in acid-base regulation of epididymis.

关 键 词：Slc26a3基因 附睾 睾丸 精子 啮齿类动物 

分 类 号：R698[医药卫生—泌尿科学]