LA系列百合‘Eyeliner’花器官组培快繁技术研究  

Floral Organs Tissue Culture and Rapid Propagation Technology of Lilium longiflorum×L.asiatic Hybrid ‘Eyeliner'

作　　者：张杰[1] 李洋[1] 孙红梅[1] 

机构地区：[1]沈阳农业大学园艺学院,设施园艺省部共建教育部重点实验室,辽宁省设施园艺重点实验室,沈阳110161

出　　处：《西北植物学报》2014年第9期1894-1899,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：辽宁省农业攻关项目(2011215003);沈阳农业大学2013年度大学生创新创业训练计划项目

摘　　要：该试验以LA系列百合‘Eyeliner’花器官为外植体,通过不定芽直接诱导和愈伤组织再分化2种途径,建立了花器官组培快繁技术体系。结果表明:以花器官为外植体污染率接近于0;4种百合花器官诱导从易到难的顺序为花梗、花丝、子房和花柱;花丝易诱导愈伤组织,花梗易诱导不定芽,最适诱导培养基均为MS+1.0mg·L-1 6-BA+0.5mg·L-1 NAA;不定芽增殖最佳培养基为MS+2.0mg·L-1 6-BA+0.2mg·L-1 NAA,愈伤组织分化最佳培养基为MS+1.5mg·L-1 6-BA+0.2mg·L-1 NAA;诱导生根最佳培养基为MS+45g·L-1蔗糖;最佳炼苗方式为将组培苗封口放置在自然环境中3d,然后开口放置在自然环境条件下2d;当试管苗鳞茎大小为3.15cm时,移栽成活率最高;移栽最佳基质为草炭∶蛭石∶珍珠岩=2∶1∶1。With the floral organs of ‘Eyeliner’ as explants,we established a rapid floral organ propagation technology via adventitious buds induction and callus redifferentiation.The results showed that：the contamination rate was 0 and the inducing abilities of different parts of floral organ were different,their sequence,from easy to hard,was pedicel,filament,ovary and style.Callus and adventitious sprouts was efficiently induced from in vitro filament as well as pedicel on MS medium appended with 1.0 mg · L 1 6-BA and 0.5 mg· L^-1 NAA.The appropriate medium for adventitious buds multiplication was MS＋2.0 mg · L 1 6 BA＋0.2 mg · L^-1 NAA,and MS supplemented with 1.5 mg · L^-1 6-BA＋0.2 mg · L^-1 NAA was the optimum medium for callus differentiation.The appropriate medium for in vitro root and bulb formations was MS＋45 g · L 1 sucrose.The optimum way of acclimatization as follows：placing the sealed plantlets in natural environment for 3 days,and then exposing them for 2 days.The highest survival rate reached when the bulb size was 3.15 cm.The suitable transplantation matrix was peat ∶ vermiculite ∶ perlite=2∶1∶1.

关 键 词：百合 花器官 组培快繁 

分 类 号：Q813.13[生物学—生物工程]