β3GnT8/β3GalT7表达载体构建及其功能  

Construction and function ananlysis of β3GnT8/β3GalT7 expression plasmid

作　　者：胡鹤娟[1] 吴士良[2] 

机构地区：[1]苏州卫生职业技术学院,江苏省215009 [2]苏州大学医学部基础医学与生物科学学院生物化学与分子生物学系

出　　处：《江苏医药》2014年第18期2112-2114,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(30670462)

摘　　要：目的探讨β1,3-N-乙酰氨基葡萄糖转移酶/β1,3半乳糖基转移酶(β3GnT8/β3GalT7)与肿瘤浸润、转移的相关性。方法构建并鉴定β3GnT8正义和反义表达载体。将空载体(A组)、正义(B组)及反义(C组)重组载体分别转染人胃癌SGC7901细胞中;另设SGC7901细胞为对照(D)组。采用RT-PCR和Western blot法分别检测四组细胞中基质金属蛋白酶-2(MMP-2)、MMP-14和转化生长因子β1(TGF-β1)的mRNA和蛋白表达。结果获得β3GnT8/β3GalT7基因正义和反义表达载体,并成功转染SGC7901细胞。D组TGF-β1、MMP-2和MMP-14表达低于B组,但高于C组。结论β3GnT8/β3GalT7表达与MMP-2、MMP-14和TGF-β1表达呈正相关,故可能参与肿瘤的发生、发展过程。Objective To investigate the relationship between βGnT8/βGalT7 and tumor invasion and metastasis. Methods The sense and antisense fragments of βGnT8 were amplified and subcloned into eukaryotic expression plasmid pEGFP-C1. The empty vector（group A）, recombinant sense plasmid（group I3） and antisense plasmid（group C） were transfected into human gastric cancer cell line SGC7901. And SGC7901 cells were taken as the controls（group D）. The mRNA and protein expressions of MMP-2, MMP-14 and TGF-β1 were detected by RT-PCR and Western blot, respectively. Results The sense and antisense expression plasmids of βGnTS/βGalT7 were successfully constructed and transfected into SGC7901 cells. The mRNA and protein expressions of MMP-2, MMP-14 and TGF-t31 in group D were lower than those in group B, but higher than those in group C. Conclusion The expression of βGnT8/βGalT7 is positively correlated with that of MMP-2, MMP-14 and TGF-β1, thus βGnT8/βGalT7 may play an important role in the development and progression of tumor.

关 键 词：β1 3-N-乙酰氨基葡萄糖转移酶/β1 3半乳糖基转移酶 基质金属蛋白酶 转化生长因子Β1 

分 类 号：Q784[生物学—分子生物学]