丹皮酚对人黑素瘤A375和M14细胞系增殖与凋亡的影响  

Effects of paeonol on the proliferation and apoptosis in human melanoma cell line A375 and M14

作　　者：陶玥[1] 张孟丽[2] 马鹏程[2] 孙建方[2] 周武庆[2] 包军[1] 

机构地区：[1]南京大学医学院附属鼓楼医院皮肤科,南京210008 [2]中国医学科学院北京协和医学院皮肤病研究所,南京210042

出　　处：《中国麻风皮肤病杂志》2014年第9期519-523,共5页China Journal of Leprosy and Skin Diseases

摘　　要：目的:确定丹皮酚对体外培养人黑素瘤A375和M14细胞增殖与凋亡的影响。方法:利用CCK8检测黑素瘤A375和M14细胞的增殖抑制率;以流式细胞仪检测细胞周期变化、Annexin-V/PI法观察细胞凋亡的变化;用Hoechst33258染色法观察细胞凋亡的形态变化。结果:细胞周期显示丹皮酚处理过的A375和M14细胞G2/M期比例均高于对照组。以0 mmol/L、1.25 mmol/L、2.5 mmol/L、5 mmol/L丹皮酚作用24 h后,A375细胞的早期凋亡率分别为3.11%±0.53%,13.74%±1.73%,25.95%±0.57%和46.44%±0.81%;M14细胞的早期凋亡率分别为1.00%±0.08%,2.00%±0.01%,2.99%±0.29%和14.73%±0.94%,呈剂量依赖性诱导A375和M14细胞凋亡。经5 mmol/L丹皮酚作用后Hoechst33258染色示凋亡细胞形态改变。结论:丹皮酚对黑素瘤A375和M14细胞均具有抑制增殖和诱导凋亡的作用。Objective：To determine the effects of paeonol on the apoptosis and proliferation in human mel-anoma cell line A375 and M14. Methods： The anti-proliferative activity of paeonol in A375 and M14 cells was investigated by CCK8 assay. After A375 and M14 cells were treated by 1.25 mmol/L, 2.5 mmol/L or 5 mmol/L paeonol for 24 h, flow cytometry was used to detect the cell cycle and Annexin V-FITC and propidi-um iodide （ PI） double staining was taken to detect the apoptosis rate of A375 and M14 cells, respectively. The morphological changes of A375 and M14 cells treated by 5 mmol/L paeonol for 24 h were observed by Ho-echest 33258 staining. Results：Compared with untreated A375 and M14 cells, an increase of cell population in G2/M phase was observed in paeonol-treated cells. After treatment of 0 mmol/L, 1. 25 mmol/L, 2. 5 mmol/L and 5 mmol/L paeonol for 24 h, the early apoptosis rate of A375 cells was 3.11%±0.53%, 13.74%±1.73%, 25. 95%± 0. 57% and 46. 44%± 0. 81%, respectively, while the rate of M14 cells Conclusion： Paeonol can inhibite proliferation and induce apoptosis of human melanoma cell line A375 and M14.

关 键 词：丹皮酚 黑素瘤 A375 M14 凋亡 

分 类 号：R285[医药卫生—中药学]