血热证银屑病患者外周血Th1、Th2和Th17的表达  

The expression of Th1/Th2/Th17 in peripheral blood of the patients with blood-heat syndrome of psoriasis

作　　者：曹筱筱[1] 徐蓉[1] 李福伦[1] 王一飞[1] 范斌[1] 李峰[1] 李斌[1] 陈洁[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院皮肤科,上海200437

出　　处：《中国麻风皮肤病杂志》2014年第9期524-526,共3页China Journal of Leprosy and Skin Diseases

基　　金：上海市自然基金(编号:11ZR1438000);上海市高级中西医结合人才培养计划(编号:ZYSNXD012RC-ZXY010);上海市卫生局项目(编号:2011XY004)

摘　　要：目的:确定血热证银屑病患者Th1/Th2/Th17主要细胞因子与银屑病血热证候的相关性。方法:分别采用流式细胞技术(FACS)及酶联免疫吸附测定法(ELISA)检测外周血单个核细胞(PMBCs)及血浆Th1/Th2/Th17细胞分泌主要细胞因子的表达。结果:血热证银屑病的CD4^+IFN-γ为26.06±11.86较正常对照12.35±6.52升高(P<0.05);CD4^+IL-4^+及CD4^+IL-17^+较正常对照稍降低(P>0.05)。IFN-γ为91.20±48.37较正常对照47.44±21.46升高,而IL-4较正常对照下降(P<0.05)。IL-17的水平两组无统计学差异。结论:血热证银屑病Th细胞免疫机制节点起主导作用的细胞亚型及细胞因子可能为n1型,主导细胞因子为IFN-γ。Objective： To determine the association between Th1/Th2/Th17 cytokines, and blood-heat syndrome of psoriasis. Methods：Flow cytometry and ELISA were applied to test Th1/Th2/Th17 cytokines in the peripheral blood of the patients with blood-heat syndrome of psoriasis and healthy controls. Results：CD4＋IFN-γ＋ was higher in the patients with blood-heat syndrome of psoriasis than in the healthy controls, with a significant difference between the two groups （P〈0.05）, but the level of CD4＋IL-4＋ and CD4＋IL-17＋ was lower than healthy controls （P〉0.05）. The level of IFN-γ was higher in the patients with blood-heat syn-drome of psoriasis than in healthy controls. The level of IL-4 was lower in the patients with blood-heat syn-drome of psoriasis than in controls （P〈0.05）. There was no significant difference in the level of IL-17 be-tween the two groups （ P〉0.05） . Conclusion：The major Th cell immune mechanism in blood-heat syndrome of psoriasis was Th1 cell and the major cytokines was IFN-γ.

关 键 词：银屑病 血热证 TH细胞 

分 类 号：R275[医药卫生—中医皮科]