Rapid point-of-use water purification using nanoscale zero valent iron(nZVI) particles  

作　　者：Jing Li Qi Chen Xinyue Li Maosheng Yao 

机构地区：[1]State Key Joint Laboratory of Environmental Simulation and Pollution Control,College of Environmental Sciences and Engineering,Peking University [2]School of Water Resources and Environment,China University of Geosciences [3]State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,People’s Liberation Army (PLA) Academy of Military Medical Sciences

出　　处：《Chinese Science Bulletin》2014年第29期3926-3934,共9页

基　　金：supported by the National Natural Science Foundation of China (21077005,20877004,41121004)

摘　　要：Nanoscale zero-valent iron(nZVI)particles are increasingly being investigated in removing aqueous contaminants.Here,we have demonstrated its inactivation and magnetic removal of bacteria and endotoxins from environmental wastewater samples.Varying dosages(10–1,000 lL)of 0–6 days aged nZVI with a concentration of 5 mg/mL for 2 mL wastewater samples were tested,and relevant removal efficiencies were determined using culturing method for bacteria and limulus amebocyte lysate(LAL)for endotoxins.The supernatants of wastewater samples after reacting with nZVI and subsequent magnetic separations were subjected to spectroscopic,qPCR and DGGE analysis.Overall,high magnetic bacterial removal efficiencies were observed up to 3–4 logs for 1 mL nZVI,while the removal efficiencies decreased sharply down to0.5 log for 10 lL nZVI.qPCR and DGGE results revealed that higher dosages of nZVI caused severe bacterial cell membrane ruptures,releasing significant amounts of DNA up to 107–108gene copies/mL when 1 mL nZVI was used.Richer DGGE patterns were observed for higher nZVI dosages.In addition,regardless of the dosages(10–1,000 lL)we have observed more than 90%removal of endotoxins from the wastewater samples.The described technology has great promise to be used as a point-of-use water purification solution for various purposes.Nanoscale zero-valent iron （nZVI） particles are increasingly being investigated in removing aqueous contaminants. Here, we have demonstrated its inactivation and magnetic removal of bacteria and endotoxins from environmental wastewater samples. Varying dosages （10-1,000 μL） of 0-6 days aged nZVI with a concentration of 5 mg/mL for 2 mL wastewater samples were tested, and relevant removal efficiencies were determined using culturing method for bacteria and limulus amebocyte lysate （LAL） for endotoxins. The supernatants of wastewater samples after reacting with nZVI and subsequent magnetic separations were subjected to spectroscopic, qPCR and DGGE analysis. Overall, high magnetic bacterial removal efficiencies were observed up to 3-4 logs for 1 mL nZVI, while the removal efficiencies 0.5 log for 10 μL nZVI. qPCR decreased sharply down to and DGGE results revealed that higher dosages of nZVI caused severe bacterial cell membrane ruptures, releasing significant amounts of DNA up to 107-108 gene copies/mL when 1 mL nZVI was used. Richer DGGE patterns were observed for higher nZVI dosages. In addition, regardless of the dosages （10-1,000μL） we have observed more than 90 % removal of endotoxins from the wastewater samples. The described technology has great promise to be used as a point-of-use water purification solution for various purposes.

关 键 词：水污染物 净化利用 零价铁 纳米 细菌细胞 废水样品 颗粒 DGGE 

分 类 号：X703[环境科学与工程—环境工程]