刺梨提取物对小鼠的抗疲劳作用  

Study on Anti Fatigue Effect of Rosa roxbughii Tratt Extract on Mice

作　　者：张硕[1] 赵梅[2] 张敏[2] 王鹏娇[2] 

机构地区：[1]中国农业大学,北京100083 [2]贵阳医学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2014年第5期675-678,共4页Journal of Guiyang Medical College

基　　金：贵州省科技厅基金[黔科合中药字(2013)5010号]

摘　　要：目的:研究刺梨提取物对小鼠的抗疲劳作用。方法:100只健康昆明种小白鼠,按雌雄、体重随机分为空白、高、中和低剂量组4组,每组25只;空白组给予生理盐水灌胃,高、中及低剂量3个组分别给予1 g/(kg·d)、2 g/(kg·d)及3 g/(kg·d)刺梨提取物灌胃;连续灌服25 d后采用小鼠游泳耐力和小鼠低压缺氧实验测定小鼠负重游泳时间及低压耐氧的死亡数;并测定运动后小鼠的血清尿素氮、血乳酸和肝糖原。结果:经灌胃小鼠不同剂量刺梨提取物25 d后,能明显延长小鼠负重游泳时间,高、中剂量组力竭游泳时间明显长于空白组,差异有统计学意义(P<0.05);高剂量组小白鼠死亡只数少于空白组,差异有统计学意义(P<0.05),小鼠死亡数量随灌服刺梨提取物剂量的增加而减少;刺梨提取物可显著增强小鼠低压耐氧时间,降低运动时血清尿素氮水平,对小鼠运动后血乳酸升高有明显的抑制作用,可使小鼠体内肝糖原的储备量增加。结论:刺梨提取物能对抗小白鼠疲劳。Objective：To investigate the anti fatigue effect of Rosa roxbughii Tratt extract on mice.Methods：100 Kunming origin mice were randomly divided into 4 groups （25 each） as empty group,high dose group,medium dose group and low dose group.Empty group were given saline,high,medium and low dose groups were given 1 g/（kg · d）、2 g/（kg · d） and 3 g/（kg · d）of Rosa roxbughii Tratt extract.After 25 days of extract feeding,the mice swimming endurance and low pressure hypoxia experiment were carried out to test the load swimming time and mortality under low pressure oxygen-resistant,and serum urea nitrogen,blood lactic acid in mice after exercise and liver glycogen were detected.Results：By feeding different dosages of extract after 25 days,it significantly extended the load swimming time,swimming time of high dose and medium dose group was significantly longer than empty group,differences were statistically different （P ＜ 0.05） ; mortality of high dose group was lower than empty group,differences were statistically different （P ＜ 0.05）.The extract could significantly reduce the death rate of low pressure oxygen tolerance in mice,reduce the serum urea nitrogen level during sports,obvious inhibitory effect were tested for elevated blood lactic acid in mice after exercise,it could increase the mice liver glycogen reserve.Conclusions：Rosa roxbughii Tratt extract has antifatigue effect on mice.

关 键 词：植物提取物 刺梨 疲劳 小鼠 血尿素氮 乳酸 肝糖原 

分 类 号：R285.5[医药卫生—中药学]