苗药防感香囊对小鼠肺组织IRAK-M、SOCS1基因和蛋白表达的影响  

Effect of Miaoyao Anti-influenza Sachet on Lab Rat Lung IRAK-M,SOCS1 Gene and Protein Expression

作　　者：李君莉[1] 张权[1] 程明亮[1] 吴君[1] 杨京[1] 张宝芳[1] 

机构地区：[1]贵阳医学院附院感染科,贵州贵阳550004

出　　处：《贵阳医学院学报》2014年第5期706-708,共3页Journal of Guiyang Medical College

摘　　要：目的:探讨苗药防感香囊对小鼠肺组织白介素1受体相关激酶M(IRAK-M)、细胞因子信号抑制因子(SOCS1)基因和蛋白表达的影响。方法:48只小鼠随机分为空白对照组(A组)、单纯香囊持续吸入组(B组)、单纯冷刺激组(C组)、香囊持续吸入加冷刺激组(D组),每组12只,分别给予相应干预,37 d后收集肺组织,采用定量PCR和免疫印迹法检测小鼠肺组织IRAK-M、SOCS1 mRNA和蛋白表达。结果:B、D组小鼠肺组织中IRAK-M mRNA表达水平低下,与A、C组比较差异有统计学意义(P<0.05);B、D组肺组织中SOCS1 mRNA表达水平低于C组(P<0.05);B组SOCS1蛋白表达水平低下,与A、C组比较差异有统计学意义(P<0.05);其余各组IRAK-M的蛋白表达水平,差异无统计学意义(P>0.05)。结论:苗药防感香囊能够下调TLR通路的负性调控因子IRAK-M、SOCS1 mRNA及SOCS1蛋白的表达水平,增强TLR通路介导的天然免疫功能。Objective：To investigate the effect of Miaoyao anti-influenza sachet （referred to as：Miao Sachet） on lab rat lung IRAK-M,SOCS1 gene and protein expression.Methods：All the rats were divided into 4 groups randomly in equal numbers,control group （Group A） ; inhaling Miao Sachet group （Group B）; cold stimulating group （Group C）; inhaling Miao Sachet and cold stimulating group （Group D）.Lung tissues were collected 37 days later,quantitative PCR and western blot were adopted to test IRAK-M,SOCS1 gene and protein expression.Results：The expression of IRAK-M and SOCS1 gene of B and D groups was lower than that of A and C groups,the differences were statistically significant （P ＜ 0.05） ; the expression of SOCS1 gene of B and D groups was lower than C group （P ＜ 0.05）,the expression of SOCS1 gene of B group was lower than A and C groups,differences were statistically significant （P ＜ 0.05） ; comparing expressions of IRAK-M protein with each other,there was no statistical significance （P ＞ 0.05）.Conclusions：Expression of IRAK-M、SOCS1 expression could be regulated by Miao Sachet with continued inhaling.

关 键 词：苗药防感香囊 蛋白激酶类 抑制蛋白类 免疫 天然 

分 类 号：R285.5[医药卫生—中药学] R286.4[医药卫生—中医学]