中国大豆花叶病毒SC7外壳蛋白基因的序列测定及其与美国株系的比较  

Sequence Analysis of the Coat Protein Gene of Chinese Soybean Mosaic Virus Strain SC7 and Comparison with Those of SMV Strains from the USA

作　　者：蔡春梅[1] 姜骁[1] 赵春梅[1] 马渐新[1] 

机构地区：[1]青岛农业大学生命科学学院山东省高校植物生物技术重点实验室,青岛266109

出　　处：《病毒学报》2014年第5期489-494,共6页Chinese Journal of Virology

基　　金：国家自然科学基金资助项目(31071444;31371647);山东省优秀中青年科学家科研奖励基金(BS2011NY006);青岛农业大学高层次人才启动基金(630908;631304)

摘　　要：为了探索大豆花叶病毒（Soybean mosaic virus,SMV）SC7外壳蛋白（Coat protein,CP）基因序列与病毒致病性作用及其与美国株系的对应关系,本研究通过反转录-聚合酶链式反应（RT-PCR）,克隆了我国SMV SC7株系的CP基因,并测定了其全序列。结果表明：CP基因长度为795bp,编码的CP蛋白为265个氨基酸。在致病性反应上,SC7的毒力比美国的毒株更强些。在分子水平上,CP基因核苷酸序列差异为4%~5%,编码的氨基酸序列差异为1%~2%。美国株系N端都存在与蚜传有关的保守基序DAG（Asp-Ala-Gl）,但在SC7为DAD（Asp-AlaAsp）。将序列信息与SMV株系在鉴别寄主上的症状反应进行综合分析发现,CP基因的同源性与SMV在鉴别寄主上的致病性反应没有明显关系。To unveil genetic variations between the predominant soybean mosaic virus （SMV） strains in Chi- na and in the USA, as well as to reveal the potential relevance between the similarity of gene sequences and the virulence of the viruses, we isolated and sequenced the coat protein （CP） gene of Chinese SMV strain SC7 by RT-PCR and compared the SC7 sequence with those of SMV strains from the USA. Analysis is showed that the CP gene of SC7 was 795 nucleotides in length and encoded 265 in amino acids＇. The CP gene of SC7 and those of the strains from the USA exhibited 4%-5 % nucleotide diversity and 1%-2 % di- versity amino acids. The conserved amino-acid sequence associated with aphid spread in the USA strains was DAG, and corresponded to DAD in SC7. The virulence of SC7 was greater than that of the SMV strains from the USA. Nevertheless, no clear relationships between sequence similarity of the CP genes from different strains and their virulence on differential hosts were found.

关 键 词：大豆花叶病毒 外壳蛋白 序列分析 致病性反应 

分 类 号：S435.39[农业科学—农业昆虫与害虫防治]