四氢嘧啶羟化酶的研究进展  

Research Process of Ectoine Hydroxylse

作　　者：张庆[1] 王青青[1] 鞠建松[1] 徐书景[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050024

出　　处：《生物技术通讯》2014年第5期719-722,733,共5页Letters in Biotechnology

基　　金：河北省自然科学基金(C2011205045);河北省留学回国人员资助项目(20100705);河北省高等学校科学技术研究青年基金(2011102);河北师范大学重点基金(L2012Z12)

摘　　要：作为相容性物质,5-羟化四氢嘧啶不仅可以调节渗透压,还可以稳定蛋白结构,在医药、生物制造和化工行业具有广阔的发展前景。四氢嘧啶羟化酶属于Fe2+与2-酮戊二酸依赖型双加氧酶超家族,主要催化四氢嘧啶生成5-羟化四氢嘧啶。我们简要介绍了四氢嘧啶羟化酶的基因来源、活性检测、蛋白结构、催化机理及活性中心等方面的研究进展。5-hydroxyeetoine is an effective compatible solute, it not only confers protection against osmotic stress, salt stress, temperature stress and dehydration, but also it has been considered for development in the pharmaceutical, bio-manufacturing and chemical industries. The eetoine hydroxylase, responsible for the conversion of ectoine to hydroxyectoine, is a member of the non-heme iron（ Ⅱ ）-containing and 2-oxoglutarate-dependent dioxygenases superfamily（EC 1.14.11）. Here, recent research on this enzyme was summarized, including its gene sources, activity detection, enzyme structure, catalytic mechanism and active centre.

关 键 词：四氢嘧啶羟化酶 四氢嘧啶 5-羟化四氢嘧啶 

分 类 号：Q55[生物学—生物化学]