短时血清培养与多聚赖氨酸对耳蜗螺旋神经节细胞活性与贴附性的作用  

Effect of Serum Culture Shortly and Polylysine in Patch Clamp Research on SGNs

作　　者：江远明[1] 唐世雄[1] 魏晓菲[2] 罗琳[2] 

机构地区：[1]宁波市第一医院耳鼻咽喉科,315010 [2]宁波大学医学院生理与药理学科系,浙江省病理生理学技术研究重点实验室,315211

出　　处：《医学研究杂志》2014年第9期124-126,共3页Journal of Medical Research

摘　　要：目的体外分离大鼠耳蜗螺旋神经节细胞(spiral ganglion neurons,SGN),探索急性分离后短时血清培养可提高耳蜗SGN的活性,同时观察多聚赖氨酸对耳蜗SGN的贴附性影响,为采取膜片钳技术研究耳蜗SGN电生理特性提供实验平台。方法采用急性分离的方法获得耳蜗SGN后,进行短时间的血清培养,同时在培养皿的底部加入多聚赖氨酸。分别比较耳蜗SGN在活性和贴附性上与未进行血清培养及未加多聚赖氨酸时的差异。结果急性分离后的耳蜗SGN,短时血清培养后比不培养的能获得更好的细胞状态,可以记录出稳定的电流曲线,并且能持续良好的细胞活性约5h,而不加血清培养的细胞,良好活性只能持续约半小时。在培养皿底加用多聚赖氨酸,可明显提高耳蜗SGN的贴附性。结论急性分离方法得到的耳蜗SGN,通过加血清短时培养及在培养皿底加多聚赖氨酸,可明显提高耳蜗SGN的活性及贴附性,增加了细胞的记录时间和封接成功率,从而有利于对耳蜗SGN做进一步电生理学的研究。Objective To study the effect of serum culture shortly and polylysine in patch clamp research on SGNs.Methods The better activity of SGN with serum culture shortly and the promoting adherence of SGN with polylysine were observed.Results The SGN with serum culture for a short time could last for at least 5 hours.It was enough for us to do some kinds of tests on the SGN.However,the SGN without serum culture just last only half an hour.The SGN with polylysine in the culture dish could hold tightly when shaking the test table,but they mostly disperse when there was no polylysine.Conclusion The SGN with serum culture for a short time can last for a long time,and hold tightly with polylysine in the dish.

关 键 词：螺旋神经节细胞 多聚赖氨酸 贴附性 

分 类 号：R76[医药卫生—耳鼻咽喉科]