鸡马立克氏病病毒编码的miRNA体内表达特征分析  

Analysis of the expression profiles of Marek's disease viruses encoded microRNAs in vivo

作　　者：郑惠文[1] 李志杰[1] 张艳萍[1] 包珂岩 郑永胜[1] 刘长军[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽免疫抑制病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2014年第10期751-754,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31201924);兽医生物技术国家重点实验室自主课题(SKLVBP201406);中央级公益性研究所基本科研业务费专项资金(0302013007)

摘　　要：最新研究发现,鸡马立克氏病病毒(MDV)基因组编码的miRNAs在肿瘤发生发展中发挥了重要的调控作用。为阐明强弱毒株编码的miRNA差异表达与病毒复制的关系,本研究采用SYBR Green荧光定量PCR方法检测了MDV CVI988疫苗株和MDV GA强毒株编码的M4-5P、M8-3P和M12-3P 3种miRNAs在肝脏和胸腺中的动态表达特征。结果表明,强弱毒株编码的这3种miRNAs在病毒感染过程中具有明显的差异性,其中GA株编码的miRNAs表达量高于CVI988株,这是由于强弱毒株在体内的复制滴度差异造成的,表明在感染前期miRNAs的表达量与病毒载量有关。该研究为进一步研究miRNAs的功能提供了实验依据。Recent studies have found that Marek＇s disease viruses （MDV） encoded microRNAs play an important role in oncogenicity. To investigate the expression of MDV encoded miRNAs in chickens infected with the virulent and vaccine strain MDV, respectively, the expressions of three MDV-encoded miRNAs M4-5P, M8-3P and M12-3P in liver and thymus infected with virulent strain GA and vaccine strain CVI988 was analyzed by quantitative RT-PCR （qRT-PCR）. The results showed that expression levels of virus encoded miRNAs was significantly higher in virulent strain GA infected chicken group than that in vaccine strain CVI988 infected group which kept in line with virus loads in the early stage of MDV infection. These data provided a basis to the further study on the mechanism of viral miRNA in establishment of MDV infection.

关 键 词：鸡马立克氏病病毒 MIRNA 荧光定量PCR 

分 类 号：S852.65[农业科学—基础兽医学]