2013年内蒙古地区蓝舌病流行情况调查  被引量:6

The epidemiological survey of bluetongue disease in Inner Mongolia in 2013

在线阅读下载全文

作  者:张胜男[1] 吕建华[1] 常建华 韩明浩 刘昆[1] 海岩[1] 刘威 吴树清[1] 

机构地区:[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古农牧科学院,内蒙古呼和浩特010031

出  处:《中国预防兽医学报》2014年第10期759-762,共4页Chinese Journal of Preventive Veterinary Medicine

基  金:国家公益性行业(农业)科研专项(201303035)

摘  要:为了解内蒙古地区蓝舌病(BT)流行情况,本研究于2013年对内蒙古存在疑似BT病例地区进行流行病学调查,收集了内蒙古6个盟市不同种类动物的抗凝血及其对应血清样品212份,采用竞争ELISA及套式RT-PCR方法对样品进行检测。竞争ELISA检测结果显示212份血清样品中有63份为BT阳性样品,13份为BT弱阳性,总阳性率为35.85%(76/212)。套式RT-PCR检测结果显示212份血液样品中有61份为BT阳性样品,总阳性率为28.77%(61/212),其中绵羊感染率为21.25%(17/80)、绒山羊感染率为35.35%(41/116)、牛感染率为18.75%(3/16)。不同的饲养方式对动物感染BT也有影响,其中圈养感染率为16.67%(1/6),散养感染率为29.13%(60/206)。本研究结果表明,内蒙古地区BT流行范围比较广泛,因此有必要加强该病的检测及预防。To investigate the prevalence of bluetongue disease (BT) in Inner Mongolia,a total of 212 blood samples were collected from ruminant livestock in different regions of Inner Mongolia in 2013,and subjected to detections by competitive ELISA (C-ELISA) and nested RT-PCR methods.The results showed that the BT positive rates of samples were 35.85% (76/212) detected by C-ELISA and 28.77% (61/212) by RT-PCR.In addition,the BT virus (BTV) infection rate was 21.25% (17/80) in sheep,35.35% (41/116) in cashmere goats,and 18.75% (3/16) in bovine.However,different feeding methods also had an impact on BTV infection.The BTV positive rates were 16.67% (1/6) and 29.13% (60/206) for livestock raising in pen-stabling and non-pen stabling,respectively.These data demonstrated that the BTV was widely prevalence in Inner Mongolia,therefore it is necessary to strengthen the detection and prevention of this disease.

关 键 词:蓝舌病 流行病学调查 竞争ELISA 套式RT-PCR 

分 类 号:S852.65[农业科学—基础兽医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象