腺病毒/甲病毒复制子嵌合载体猪瘟疫苗rAdV-SFV-E2最早提供完全攻毒保护时间的研究  

First onset of full protection against lethal classical swine fever virus challenge with a single immunization of rAdV-SFV-E2

作　　者：袁晋[1] 孙元[2] 王春花[2] 秦华洋 丛鑫[2] 李素[2] 罗玉子[2] 刘磊[1] 仇华吉[2] 

机构地区：[1]甘肃农业大学动物医学学院,甘肃兰州730000 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2014年第10期792-796,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863计划项目(2011AA10A208);兽医生物技术国家重点实验室基本科研业务费项目(SKLVBP201317-2)

摘　　要：本实验室前期构建了腺病毒/甲病毒复制子嵌合载体猪瘟疫苗rAdV-SFV-E2,其免疫效力与商品化猪瘟C株疫苗相同,并具有鉴别检测特性。为进一步确定该嵌合载体猪瘟疫苗最早提供完全攻毒保护的时间,本研究将rAdV-SFV-E2免疫健康仔猪(n=5),免疫后于不同时间点采用猪瘟病毒(CSFV)强毒石门株进行攻毒,并检测其抗体应答、临床症状、病毒血症、病理学及组织病理学变化等。结果显示,rAdV-SFV-E2免疫后1周和2周,分别能对致死性CSFV强毒的攻击提供部分保护(3/5存活)和不完全保护(全部存活,但出现了短暂的发热和低水平的病毒血症);免疫后3周和4周,能够完全抵抗致死性CSFV强毒的攻击,表现为无病毒血症,无临床症状,无病理变化和组织病理学变化。该研究进一步证实rAdV-SFV-E2能够作为一种新型猪瘟标记疫苗,在猪瘟的紧急防控中能够起到一定的作用。The adenovirus/alphavirus replicon chimeric vector-based vaccine rAdV-SFV-E2 against classical swine fever （CSF） was constructed in our previous study,which showed an equal efficacy to the commercial CSF vaccine of C-strain and possessed a unique characteristic of differentiating infected from vaccinated animals （DIVA）.To determinate the first onset of full protection provided by rAdV-SFV-E2,groups of pigs （n=5） were immunized once intramuscularly with 107 TCID50 rAdV-SFV-E2 and challenged with the highly virulent CSFV Shimen strain at different weeks post-immunization （WPI）.The results showed that the rAdV-SFV-E2 immunized pigs were completely protected from the lethal challenge at 3 or 4 WPI,without fever,viremia or pathological and histopathological lesions.However,rAdV-SFV-E2 provided only partial （3/5 survived） or incomplete （all survived,but showed short-term fever and viremia） protection for the immunized pigs when challenged at 1 or 2 WPI,respectively.These results demonstrate that rAdV-SFV-E2 is a promising DIVA vaccine and has a potential to be used as a safe and efficient candidate vaccine against CSF.

关 键 词：猪瘟 嵌合载体疫苗 最早保护时间 

分 类 号：S852.65[农业科学—基础兽医学]