实验性胆汁反流性胃炎大鼠胃黏膜改变及亮菌口服液的干预作用  

Changes in gastric mucosa of rats with experimental bile reflux gastritis: Interventional effects of Liangjun Oral Liquid

作　　者：王冬青[1] 丁西平[1] 殷实 

机构地区：[1]安徽医科大学附属省立医院消化内科,安徽省合肥市230001 [2]安徽医科大学附属省立医院老年病科,安徽省合肥市230001

出　　处：《世界华人消化杂志》2014年第20期2875-2880,共6页World Chinese Journal of Digestology

基　　金：2012安徽省科技厅年度重点基金资助项目;No.12070403055~~

摘　　要：目的:观察实验性胆汁反流性胃炎(bile refluxgastritis,BRG)大鼠胃黏膜的病理变化、黏膜环氧化酶2(cyclooxygenase 2,COX-2)表达的改变及亮菌口服液对其干预作用.方法:将40只大鼠随机分为4组:BRG组、亮菌口服液干预组、亮菌口服液组及空白对照组.采用自制反流液灌胃建立实验性BRG大鼠模型,光电镜观察各组大鼠胃黏膜的改变及免疫组织化学法检测胃黏膜COX-2表达改变.结果:BRG模型组大鼠病理切片胃黏膜下可见重度炎症占60%(24/40),而发生轻度肠上皮化生及异型增生大鼠均占20%(8/40),同时胃黏膜中COX-2表达强阳性率为70%(7/10),与模型组比较,亮菌口服液干预组可显著改善大鼠胃黏膜病理变化及COX-2表达(P<0.05).大鼠出现重度炎症仅占20%(8/40),发生轻度肠化生及异型增生均下降为5%(2/40),且COX-2表达强阳性率为10%(1/10).亮菌口服液组及正常对照组胃黏膜未见炎症及其他病理改变,COX-2为胃黏膜仅微量表达,两组无明显差异.结论:胆汁反流液可导致大鼠胃黏膜发生炎症、肠上皮化生及异型增生,而亮菌口服液可通过降低胃黏膜COX-2含量,减轻灌流液对胃黏膜的损害,发挥胃黏膜的保护作用.AIM： To observe the pathologic changes and the expression of cyclooxygenase-2（COX-2） in gastric mucosa of rats with experimental bile reflux gastritis（BRG） and to assess the interventional effects of Liangjun Oral Liquid. METHODS： Forty rats were divided into four groups： Ⅰ（BRG model）, Ⅱ（BRG model treated with Liangjun Oral Liquid 1 mL/kg）, Ⅲ（normal rats treated with Liangjun Oral Liquid 1 mL/kg） and Ⅳ（normal controls）. BRG was induced using self-made reflux liquid. The changes in thegastric mucosa of rats in each group were observed by optical microscopy and transmission electron microscopy（TEM）. The expression of COX-2 protein was evaluated by immunohisto-chemistry（IHC）. RESULTS： In group Ⅰ, 60% of rats had severe inflammation in the gastric mucosa, 20% had mild atypical hyperplasia, 20% had intestinal metaplasia; 70% had strong COX-2 protein expression. Compared with group Ⅰ, the pathological changes were improved and the expression of COX-2 was decreased（P 0.05） in group Ⅱ： 20% of rats had severe inflammation in the gastric mucosa, 5% had mild atypical hyperplasia, 5% had intestinal metaplasia; 10% had strong COX-2 protein expression. No pathologic changes were found in the gastric mucosa of rats in groups Ⅲ and Ⅳ, and the expression of COX-2 protein was almost un-detectable and there were no significant differences between the two groups. CONCLUSION： Liangjun Oral Liquid can re-lieve the gastric mucosal injury in rats with experimental bile reflux gastritis by decreasing the expression of COX-2.

关 键 词：实验性胆汁反流性胃炎 亮菌口服液 环氧化酶2 

分 类 号：R573.3[医药卫生—消化系统]