疏肝和胃汤对抑郁模型大鼠延髓、脊髓及胃黏膜组织5-羟色胺表达的影响  

Effect of Shugan Hewei decoction on expression of 5-hydroxytryptamine in the medulla oblongata, spinal cord and gastric mucosa of depression rats

作　　者：岳滢滢[1] 刘松林[2] 邢颖[1] 李明珠[1] 李家庚[1] 程方平[1] 

机构地区：[1]湖北中医药大学中医临床学院,湖北省武汉市430060 [2]湖北中医药大学中医临床学院伤寒教研室,湖北省武汉市430060

出　　处：《世界华人消化杂志》2014年第22期3251-3258,共8页World Chinese Journal of Digestology

基　　金：教育部新世纪优秀人才支持计划基金资助项目;No.NCET-11-0963;国家自然科学基金资助项目;No.81072720~~

摘　　要：目的:观察疏肝和胃汤对抑郁模型大鼠延髓、脊髓及胃黏膜组织5-羟色胺(5-hydroxytryptamine,5-HT)表达的影响,部分揭示疏肝和胃汤改善抑郁状态及胃肠功能作用中的5-HT调节机制.方法:100只大鼠随机分成生理盐水组、模型组、百优解组及疏肝和胃汤大、小剂量组.采用慢性心理应激加孤养法制作抑郁模型,共计造模4 wk.在第3周,百优解组及疏肝和胃汤大、小剂量组分别予以0.36mg/(kg·d)、20、10 g/(kg·d)体质量给药,生理盐水组及模型组给予等体积生理盐水,每天分2次灌胃.在第4周结束后,使用内固定法取大鼠延髓迷走神经背核部位的脑、胸髓(T6-T8,根据脊神经定位)及胃黏膜组织,应用免疫组织化学技术检测上述部位5-HT的表达.结果:模型组大鼠延髓组织5-HT含量较生理盐水组显著下降(3314.46±757.47 vs10050.01±472.82),差异具有显著统计学意义(P<0.01);模型组大鼠脊髓与胃黏膜组织5-HT含量较生理盐水组显著上升(7014.51±628.93 vs 4135.62±148.01、4400.12±315.78vs 3614.59±210.54),差异具有显著统计学意义(P<0.01).治疗2 wk后,疏肝和胃汤大、小剂量组和百优解组大鼠延髓中5-HT含量与模型组比较显著增加(9556.06±406.73 vs 3314.46±757.47、7800.91±264.37 vs 3314.46±757.47、9770.94±339.23 v s 3314.46±757.47),差异具有显著统计学意义(P<0.01),脊髓与胃黏膜中5-HT含量与模型组比较显著下降(4487.24±160.55 vs 7014.51±628.93、5667.02±294.84 v s 7014.51±628.93、4908.46±129.58 v s 7014.51±628.93、3736.45±242.36 v s 4400.12±315.78、3978.35±355.96 v s 4400.12±315.78、3826.96±474.88 vs 4400.12±315.78),差异具有统计学意义(P<0.05或P<0.01).其中,疏肝和胃汤大剂量组与百优解组延髓中5-HT含量比较,差异无统计学意义(P>0.05),而疏肝和胃汤大剂量降低脊髓与胃黏膜中5-HT含量的作用较百优解更为明显(4487.24±160.55 vs 4908.46±129.58、3736.45±242.36vs 3826.96±474.88),差异具有统�AIM： To observe the effect of Shugan Hewei de- coction （SHD） on the expression of 5-hydroxy- tryptamine （5-HT） in the brain （medulla oblon-gata）, spinal cord and gastric mucosa of depres- sion rats, and to explore the possible mechanism for SHD to relieve depression and improve gas- trointestinal function.METHODS： One hundred Wistar rats were randomized into a normal saline group, a model group, a fluoxetine group, high- and low-dosage SHD groups, with 20 rats in each group. Depression was induced with chronic unpredictable mild stress （CUMS） and separa- tion for 4 wk. From the 3r~ week, rats in the fluoxetine group, high- and low-dosage SHD groups were given fluoxetine [0.36 mg/（kg . d）], high- and low-dosage SHD [20 and 10 g/（kg.d）] by intragastric administration, twice a day, respectively. The normal saline group and model group were given equal volume of physiological saline. The treatment lasted 14 days. At the end of the experiment, the inter- nal fixation method was used to take medulla oblgngata, spinal cord （T6-T8） and gastric mu- cosa tissues. Immunohistochemistry （IHC） was used to detect the expression of 5-HT in the above tissues.RESULTS： The content of 5-HT decreased significantly in the medulla oblongata of de- pression rats compared with the normal saline group （3314.46± 757.47 vs 10050.01±472.82, P 〈 0.01）. The content of 5-HT increased signifi- cantly in the spinal cord and gastric mucosa of depression rats compared with the normal sa- line group （7014.51 ± 628.93 vs 4135.62 ± 148.01, 4400.12 ± 315.78 vs 3614.59 ±210.54, P 〈 0.01 for both）. After 2 wk of treatment, the content of 5-HT was raised significantly in the medulla oblongata of rats in the high-, low-dosage SHD groups and fluoxetine group compared with the model group （9556.06 ± 406.73 vs 3314.46 ± 757.47, 7800.91± 264.37 vs 3314.46 ±757.47, 9770.94± 339.23 vs 3314.46 ±757.47, P 〈 0.05 or P 〈 0.01）, while the content of 5-HT was signifi- cantly reduced in the spinal co

关 键 词：抑郁症 延髓 脊髓 胃黏膜 疏肝和胃汤 5-羟色胺 

分 类 号：R285.5[医药卫生—中药学]