两株西沙群岛野生诺尼种子内生酵母CICC32991与CICC32993的多相分类鉴定  

Identification on two yeasts CICC32991 and CICC32993 isolated from Paracel Islands wild Noni( Morinda citrifolia L. ) Seeds

作　　者：白飞荣 刘洋[1] 姚粟[1] 李辉[1] 谭望桥 程池[1] 

机构地区：[1]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100015 [2]海南诺尼生物工程开发有限公司,海口570125

出　　处：《中国真菌学杂志》2014年第4期199-202,共4页Chinese Journal of Mycology

基　　金：国家自然科学基金(31300008);中国食品发酵工业研究院科技发展基金(博士基金)(2012KJFZ-BS-01);北京市科技新星计划(Z141105001814095);国家微生物资源平台专项(NIMR2014-4)

摘　　要：目的通过多相鉴定方法对分离自我国西沙群岛野生诺尼种子内的两株酵母(CICC32991,CICC32993)进行准确鉴定。方法包括形态学观察试验、生理生化特征试验、以及核糖体DNA序列系统发育学分析等技术。结果形态学和生理生化试验结果显示CICC32991为Eremothecium sp.,CICC32993为Kodamaea ohmeri;系统发育分析显示CICC32991为Eremothecium coryli,CICC32993为Kodamaea ohmeri。结论综合各试验结果表明菌株CICC32991为Eremothecium coryli;CICC32993为奥默柯达菌(Kodamaea ohmeri)。Objective To distinguish the two yeasts( CICC32991,CICC32993) isolated from Paracel Islands wild Noni( Morinda citrifolia L.) seeds by the polyphasic taxonomy methods. Methods This study included morphological,physiological and biochemical characteristics of the test,as well as ribosomal DNA sequence phylogenetic analysis technology. Results Morphological and physiological test results showed the strain CICC32991 as Eremothecium sp. and CICC32993 as Kodamaea ohmeri. Phylogenetic analysis results showed CICC32991 as Eremothecium coryli and CICC32993 as Kodamaea ohmeri. Conclusion The results certified the strain CICC32991 as Eremothecium coryli and the strain CICC32993 as Kodamaea ohmeri.

关 键 词：西沙群岛 诺尼 多相鉴定 CICC32991 CICC32993 

分 类 号：Q939.5[生物学—微生物学]