小鼠脑微血管内皮细胞与隐球菌作用前后基因表达谱分析  

Gene expression profilinganalysis of mouse brain microvascular endothelial cells incubated with Cryptococcus neoformans

作　　者：钟彬[1,2] 李平[1,2,3] 聂舒[1,2] 朱红梅[1,2] 温海[1,2] 

机构地区：[1]上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室 [2]第二军医大学长征医院皮肤科,上海200003 [3]国防科学技术大学军干所卫生所,长沙410074

出　　处：《中国真菌学杂志》2014年第4期203-206,共4页Chinese Journal of Mycology

基　　金：国家自然科学基金(31270181;30870106;81071336)

摘　　要：目的比较小鼠脑微血管内皮细胞系bEnd.3细胞与隐球菌作用前后基因表达谱的变化,为隐球菌的嗜中枢性研究提供新的线索。方法采用基因芯片法比较bEnd.3细胞与不同血清型隐球菌作用前后基因表达谱的变化,并进一步通过荧光定量PCR的方法对某些重要基因的变化加以验证。结果我们对bEnd.3与隐球菌作用前后基因表达进行了对比,共获得差异基因383条,其中263条基因表达下降,120条基因表达上升,并根据比较结果选取了黏附分子CDH10及硒转运蛋白SELENBP1两个基因进行荧光定量PCR验证,结果与芯片结果一致。发现bEnd.3与隐球菌作用后CDH10表达明显下降,而SELENBP1表达明显上升。结论隐球菌能引起脑血管内皮细胞黏附分子CDH10表达下降及硒结合蛋白selenbp1表达上升,这可能与其侵袭血脑屏障有关。而SELENBP 1的表达上升可能与神经系统症状有关。Objective To dissect genomic changes in gene expression of mouse brain microvascular endothelial cells infected by Cryptococcus neoformans for providing new clues of fungal predilection for the central nervous system. Methods By comparing the gene expression profiles of bEnd. 3 cells before and after infected by Cryptococcus neoformans of different serotypes, the changes of some important genes were found and then validated by RT-PCR. Results After comparison, we got 383 differentially expressed genes ,including 263 down-regulated and 120 up-regulated ones. And then we selected adhesion molecule gene CDHIO and seleni- um binding protein 1 gene SELENBP 1 and validated their expression levels by RT-PCR . The results were in line with the microar- ray data. Conclusion Cryptococcus neoformans caused down-regulation of adhesion molecule CDHIO and up-regulation of SELENBP1 in brain microvascular endothelial cell might contribute to the yeast~ invasion to host blood-brain barrier （BBB）.

关 键 词：脑微血管内皮细胞 基因芯片 新生隐球菌 

分 类 号：R379.5[医药卫生—病原生物学]