P2Y13受体激活促进脊髓背角小胶质细胞钙浓度升高  

Rapid elevation of calcium concentration in cultured dorsal spinal cord microglia by ADP

作　　者：王高霞 朱萍[1] 黄晓丽[1] 田虹[1] 刘晓红[1] 曾俊伟[1] 

机构地区：[1]遵义医学院生理学教研室,贵州省麻醉与器官功能保护重点实验室,遵义563000

出　　处：《神经解剖学杂志》2014年第5期529-534,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金资助(31000497,31460266);教育部科学技术重点项目(212154);教育部新世纪优秀人才支持计划(NCET-13-1070);遵义医学院重点学科项目(XZXK-20120702)

摘　　要：目的:观察ADP对培养的脊髓背角小胶质细胞内Ca2+浓度([Ca2+]i)的影响及作用机制。方法:培养纯化新生SD大鼠脊髓背角小胶质细胞,免疫组化观察P2Y13受体表达,激光共聚焦显微镜检测ADP作用下,小胶质细胞[Ca2+]i的变化。结果:大鼠脊髓背角小胶质细胞表达P2Y13受体,ADP可导致培养的脊髓背角小胶质细胞[Ca2+]i快速增高,且呈现剂量依赖性;P2Y13受体拮抗剂MRS2211(100μmol/L)能基本阻断ADP的作用,而P2Y1受体拮抗剂MRS2179(100μmol/L)、P2Y12受体拮抗剂MRS2395(100μmol/L)均不影响ADP致小胶质细胞[Ca2+]i升高的效应。结论:ADP可能通过P2Y13受体途径,导致培养的脊髓背角小胶质细胞[Ca2+]快速升高。Objective: To explore whether ADP has an effect on the intracellular calcium concentration([Ca2 +]i) in cultured dorsal spinal cord microglia and the relative mechanism. Methods: A cell culture model of SD rat spinal cord microglia was used. The expression of P2Y13 receptor in cultured dorsal spinal cord microglia were observed by using immunohistochemical staining. Changes of intracellular calcium fluorescence intensity of microglia were measured by laser scanning confocal microscopy. Results: Immunofluorescence labeling showed that most of Iba-1 positive microglia was also stained for the P2Y13 receptor in cultured spinal cord microglia. ADP induced [Ca2 +]i increased in cultured dorsal spinal cord microglia within several minutes. The rapid elevation of [Ca2 +]i in microglia by ADP was concentration-dependent and could be blocked by P2Y1 receptor antagonist MRS2179(100 μmol /L) and P2Y13 receptor antagonist MRS2211(100 μmol /L),but not by P2Y12 receptor antagonist MRS2395(100 μmol /L). Conclusion: ADP induces[Ca2 +]i increase possibly mediated by PZY13 receptor in spinal cord microglia cells in vitro.

关 键 词：ADP 脊髓 小胶质细胞 钙 

分 类 号：R965[医药卫生—药理学]