三七总皂甙对大鼠脑出血后神经可塑性的影响  

The influence of PNS on the neuroplasticity after cerebral hemorrhage in rat

作　　者：王登科[1,2] 朱继红[1,2] 戴新文[1,2] 贺岭风[1,2] 孙征[2] 刘海洋[2] 

机构地区：[1]宁夏师范学院医学院,固原756000 [2]宁夏医科大学,银川750004

出　　处：《神经解剖学杂志》2014年第5期569-574,共6页Chinese Journal of Neuroanatomy

基　　金：宁夏师范学院科研项目(YB201346);自治区卫生厅重点科研计划资助(NW2011010)

摘　　要：目的:探讨三七总皂甙对大鼠脑出血后神经可塑性的影响。方法:制作脑出血模型;将120只大鼠随机分成假手术组、模型组、三七总皂甙治疗组;免疫组化检测Nestin、Shank1表达;电镜观察突触超微结构并定量分析;对大鼠进行神经功能评分。结果:假手术组超微结构正常,模型组神经突触及细胞器溶解破坏,治疗组突触结构趋于正常,与模型组比较突触数量、界面曲率及突触后致密区均增大,突触间隙变窄,差异有统计学意义(P<0.01)。与模型组比较,治疗组Shank1及Nestin蛋白阳性表达升高,差异有统计学意义(P<0.01)。假手术组神经功能基本正常;模型组评分升高;与模型组比较,治疗组评分降低,差异有统计学意义(P<0.01)。结论:三七总皂甙可增强神经可塑性,保护大鼠神经功能。Objective： Study of the effect of PNS on neural plasticity after cerebral hemorrhage in rats. Method： Pro- duction models of cerebral hemorrhage. 120 rats were randomly divided into sham operation, model and treatment groups. The ultrastructure of nervous tissue was observed by transmission electron microscope, and then dothe quantitative analg- sis. The expression of Shank1 and Nestin protein were detected by immunohistochemistry. Evaluate the neurolcgical scores of rats. Results： The ultrastructure of nervous tissue was normal in the sham operation group. Nerve synapse and organelles were destroyed in the model group. The synapse structure tended to be normal in the treatment group. The number of synapses, the synaptic interface curvature and postsynaptic dense areas were increased, the gap of synapse nar-rowed obviously in treatment groups, and it is significant in statistics compared with the model group（P 〈 0.01 ）. The ex-pression of Shank1 and nestin protein increased in the treatment group, and it is significant in statistics compared with the model group（P 〈 0.01 ）. The nerve function was normal in the sham opertaion group. The neurological scores of treat-ment group was significantly decreased（P 〈 0.01 ）. Conclusion： The PNS can strengthen the plasticity and protect the nerve function of rat.

关 键 词：脑出血 三七总皂甙 神经可塑性 突触 大鼠 

分 类 号：R285.5[医药卫生—中药学]