布鲁氏菌病血清学Brucellacapt和iELISA检测方法的比较  

Comparison of Brucellacapt and iELISA in serological diagnosis of brucellosis

作　　者：赵娜[1] 赵赤鸿[2] 荣蓉[2] 肖培[1] 孙岩[1,3] 王娜[1,3] 崔步云[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,北京102206 [2]中国疾病预防控制中心,北京102206 [3]内蒙古农业大学,呼和浩特010018

出　　处：《中国人兽共患病学报》2014年第10期1045-1047,1051,共4页Chinese Journal of Zoonoses

基　　金：国家卫生计生委公益性行业科研专项(No.201302006)资助~~

摘　　要：目的探索Brucellacapt方法对于我国布鲁氏菌病的诊断价值。方法对来门诊就诊的120例就诊者,使用RBPT和SAT方法结合临床表现等诊断布鲁氏菌病病人75例,非布鲁氏菌病病人45例。对全部120份就诊者血清进行Brucellacapt和iELISA检测并比较分析其在诊断中的意义。结果 Brucellacapt检测方法的灵敏度、特异度、符合率、Kappa值和ROC曲线下面积分别为82.7%、88.9%、85.0%、0.69、0.86,而iELISA方法的结果分别为90.7%、64.4%、80.8%、0.57、0.78。结论 Brucellacapt检测方法的特异度、符合率、Kappa值和ROC曲线下面积均高于iELISA检测方法,而iELISA检测方法的灵敏度较高。Brucellacapt is a new serological test based on immunocapture-agglutination technique that has been used in many countries, but there was no related article reported in China. The value of the Brucellacapt method in diagnosing human brucellosis was evaluated in this study. Among 120 suspected patients, 75 patients and 45 negatives were diagnosed by SAT and RBPT method combination with their clinical symptoms. Sera from all 120 people were tested by the method of Brucella- capt and iELISA, and the results were, consequently, analyzed and compared. It showed that sensitivity, specificity, consis- tency rate, Kappa value, and area Uflder ROC curve were found to be 82.7%, 88.9%, 85.0%, 0.69, and 0.86, respectively for Brucellacapt, whereas they were found to be 90.7%, 64.4%, 80.8%, 0.57, and 0.78 for iELISA. In conclusion, specific- ity, consistency rate, Kappa value, and area under ROC curve were higher in Brucellacapt method than that in iELISA. How- ever, the sensitivity of iELISA is higher.

关 键 词：布鲁氏菌病 诊断 Brucellacapt IELISA 

分 类 号：R378.5[医药卫生—病原生物学]