蝮蛇毒血小板抑制因子对家兔动脉血栓形成的影响  

Effects of platelet inhibitor from Agkistrodon halys venom on artery thrombosis in rabbit

作　　者：季娜[1] 张根葆[1,2] 黄璐[1] 王斐[1] 张娅[1] 吴娟[1] 

机构地区：[1]皖南医学院病理生理学教研室 [2]皖南医学院蛇毒研究所

出　　处：《中国临床药理学与治疗学》2014年第9期987-990,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究蝮蛇毒血小板抑制因子(AHVPI)对实验家兔动脉血栓形成的影响及其可能机制。方法:30只雄性家兔随机分为3组(n=10),即对照组(假手术组),模型组,AHV-PI组(0.2mg/kg)。以70%FeCl3溶液化学损伤的方法制备家兔颈动脉血栓模型。1h后经另一侧颈动脉采血,用双通道凝血仪测定家兔APTT、PT与TT以及血浆纤维蛋白原(FIB)含量,酶联免疫吸附法(ELISA)测定各血浆中纤溶酶水平。结果:与对照组相比较,模型组APTT、PT与TT均明显缩短(P<0.05),血浆中FIB含量增高(P<0.05),纤溶酶含量降低(P<0.05);与模型组相比,AHV-PI组APTT、TT延长(P<0.05),PT无明显改变(P>0.05),血浆中FIB含量降低(P<0.05),纤溶酶含量进一步降低(P<0.05)。病理切片显示AHV-PI组颈动脉内膜相对比较平滑,管腔内未见血栓形成。结论:AHV-PI抑制动脉血栓形成,其机制可能与纤溶酶激活有关。AIM： To explore the effects and possible mechanism of platelet inhibitor from Agkistrodon halys venom （AHV-PI） on artery thrombosis in rabbit. METHODS： Thirty rabbits were divided into control group, artery thrombosis model group and AHV-PI （0.2 mg/ kg） group （n：10） randomly. The model of rabbits carotid artery thrombosis was induced by infiltering 70% FeC13. The blood was taken from another carotid artery after, 1 hour. Automatic coagulation analyzer was used to determined the APTT, PT, TT and the content of fibrinogen （FIB）. The plasma content of plasmin was determined by Enzyme-Linked Immunosorbnent Assay （ELISA）. RESULTS ： Compared with control group, significantly shortened APTT, PT, TT and reduced plasmin level were observed in artery thrombosis model group （P〈0.05）, while the level of FIB in plasma was increased （P〈 0.05）. Obviously prolonged APTT and TT, reduced FIB （P〉0.05） and plasmin level were discovered in AHV-PI group as compared with model group, while PT had no difference （P〈 0.05）. CONCLUSION. AHV-PI can prevent the artery thrombosis. The mechanism may be related to plasminogen activation.

关 键 词：蝮蛇毒 动脉血栓 纤溶酶原激活 

分 类 号：R965.2[医药卫生—药理学]