蒺藜提取物治疗2型糖尿病药效及其机制研究  

The efficacy of Fructus Tribuli extract JL201on type 2diabetes and its potential mechanisms

作　　者：赵保胜[1] 李朋收[2,3,4] 张舒媛[5] 李春娜[2,3,4] 刘洋洋[2,3,4] 时晓娟[2,3,4] 徐暾海[2,3,4] 刘铜华[3,4] 

机构地区：[1]北京中医药大学科研实验中心,北京100029 [2]北京中医药大学中药学院,北京100029 [3]北京中医药大学中医养生学教育部重点实验室,北京100029 [4]北京中医药大学中医养生学北京市重点实验室,北京100029 [5]北京中医药大学管理学院,北京100029

出　　处：《中国临床药理学与治疗学》2014年第9期1006-1010,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金面上项目(81374027);国际科技合作计划(2010DFB33260);北京中医药大学创新团队项目(2011-CXTD19);北京中医药大学中青年教师资助项目(2013-JYBZZ-JS-131)

摘　　要：目的:观察蒺藜提取物(NO.JL201)对自发性2型糖尿病模型KKAy小鼠空腹血糖(FPG)、血胰岛素(Ins)、TNF-α、IFN-β含量及其胰岛细胞TLR3、TLR4mRNA含量的影响,初步探讨其可能降糖机制。方法:KKAy小鼠随机分为模型组、JL201大(20mg/kg BW)、中(10mg/kg BW)、小(5mg/kg BW)剂量组和阳性药二甲双胍组(400mg/kg BW)。每周测定体质量,食、水量和FPG。给药4周后取血,测定小鼠FPG和Ins,计算小鼠Ins敏感性,Real time-PCR法测定胰岛细胞TLR2、TLR4 mRNA含量。结果:JL201不同剂量不同程度地减少了小鼠的进食、饮水量,明显降低KKAy小鼠的FPG(P<0.05或P<0.01)、降低血清Ins含量(P<0.05或P<0.01),明显升高KKAy小鼠胰岛素敏感性(P<0.05),减少TNF-α、IFN-β的血中分泌量。JL201同时可降低胰岛细胞TLR2、TLR4mRNA含量。结论:JL201有较好的降血糖、增强胰岛素敏感性等作用,并能明显改善多饮、多食的临床症状,其作用可能与降低胰岛细胞TLR2、TLR4mRNA含量、抑制炎症有关。AIM： To observe the effect of Fructus Tribuli extract （NO. JL201） on the concentration of fasting plasma glucose, blood insulin, TNF-α, IFN-β and that of TLR3, TLR4 mRNA in islet cell on spontaneous type 2 diabetes KKAy mice, and explore its potential hypoglycemic mechanisms. METHODS： Mice were randomly allocated into 5 groups： control group, JL201 high -dosage group （20 mg/kg BW）, JL201 middle -dosage group （10 mg/kg BW）, JL201 low-dosage group （5 mg/kg BW）, and positive drug metformin group （400 mg/kg BW）. Body weights, food and water intake and fasting plasma glucose （FPG） were measured weekly. Mice were sacrificed after 4 weeks administration to obtain the blood sample and measured the concentration of FPG and insulin （Ins）, and calculated the sensitivity of Ins in mice. The concentrations of TLR2, TLR4 mRNA in Islet cell were measured using realtime- PCR method. RESULTS：Different concentration of JL201 reduced the intake of food and water respectively. In addition, the concentration FPG decreased significantly （P〈 0.05 or P〈0.01） and the concentration of serum Ins also lowed （P〈0.05 or P〈0.01）. However, the sensitivity of Ins increased significantly （P〈0.05） and the secretion of TNF-α, IFN-β reduced. At the same time, JL201 could decrease the concentration of TLR2, TLR4 mRNA spon- taneously. CONCLUSION. JL201 has beneficial effects on hypoglycemic and strengthening the sensitivity of Ins. Besides, it also can improve polydipsia and polyphagia clinical symptoms. The potential beneficial effects may be associated with decreasing islet cell TLR2, TLR4 mRNA levels and inhibiting inflammation.

关 键 词：蒺藜 2型糖尿病 药效 机制研究 

分 类 号：R965.2[医药卫生—药理学]