基于ITS序列探讨沙棘属植物的系统发育关系  

Phylogenetic relationships of seabuckthorn based on ITS sequences

作　　者：马玉花[1] 冶贵生[1] 向前胜[1] 高英[1] 杨春江[1] 魏国良[1] 宋维秀[1] 

机构地区：[1]青海大学农牧学院,西宁810016

出　　处：《应用生态学报》2014年第10期2985-2990,共6页Chinese Journal of Applied Ecology

基　　金：中国科学院"西部之光"人才培养计划项目资助

摘　　要：对分布于青海境内的中国沙棘、肋果沙棘和西藏沙棘的ITS区进行扩增和序列分析,并以胡颓子科胡颓子属沙枣为外类群,对胡颓子科沙棘属15种植物的ITS序列进行聚类分析,探讨沙棘属各植物的亲缘关系.结果表明：3种沙棘属植物的ITS区长度为600-605 bp,其中,ITS-1区为201-203 bp,5.8S为166-167 bp,ITS-2区为232-236 bp.核苷酸分析显示,3种沙棘属植物的ITS区存在丰富的变异位点.聚类分析表明,棱果沙棘种的2个亚种——棱果沙棘和理塘沙棘为2个不同种,江孜沙棘与柳叶沙棘之间的亲缘关系较近,而与中国沙棘亲缘关系较远;沙棘种下9个亚种间的聚类结果与形态学分类差异较大.Sequences of the internal transcribed spacer （ITS） was compared in three seabuckthrons （ Hippophae rhamnoides subsp, sinensis, H. tibetana and H. neurocarpa ） distributed in Qinghai Province, then the systematic positions of 15 seabuckthron samples were analyzed with Elaeagnaceae angustifolia being outgroup. The results showed that ITS sequences in three seabuckthron species varied in length from 600 to 605 bp. ITS-1, 5.8S and ITS-2 varied from 201 to 203 bp, 166 to 167 bp and 232 to 236 bp, respectively. The sequence divergence among three seabuckthorn species was also remarkably high. Cluster analysis based on ITS indicated that H. gonicocarpa subsp. litangensis and H. gonicocarpa subsp, goniocarpa were distinct and could be classified as H. gonic- ocarpa and H. litangensis. H. gyantsensis had the closest genetic relationship with H. salicifolia and the distant relationship with H. rhamnoides subsp, sinensis. The positions of nine subspecies of H. rhamnoides based on morphological classification were different from the results of ITS.

关 键 词：沙棘 ITS 亲缘关系 

分 类 号：S793.6[农业科学—林木遗传育种]