土壤外源金霉素污染对蚯蚓生长发育的生态毒性效应  

Ecotoxicological effects of chlorotetracycline on earthworm in soil

作　　者：纪占华[1,2] 安婧[1] 肖明月[3] 魏树和[1] 台培东[1] 律泽[4] 

机构地区：[1]中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学资源与环境学院,北京100049 [3]辽宁石油化工大学化学化工与环境学部,辽宁抚顺113001 [4]东北大学资源与土木工程学院,沈阳110004

出　　处：《应用生态学报》2014年第10期3011-3016,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(40930739;41001340;21277150;31370523);国家环境保护公益性行业科研专项(201209030);辽宁环境科研教育123工程项目(CEPF2012-123-2-1)资助

摘　　要：采用赤子爱胜蚓作为受试生物,研究了土壤金霉素污染对蚯蚓生长发育的慢性毒性效应.结果表明:暴露7 d时,1、10、100 mg·kg-1金霉素对蚯蚓体质量均无显著影响,暴露21d后,10、100 mg·kg-1金霉素对蚯蚓体质量产生显著抑制作用;1、10、100 mg·kg-1金霉素均诱导了蚯蚓体内可溶性蛋白含量的增加,且暴露浓度越高,诱导效应越强;1、10、100mg·kg-1金霉素胁迫下蚯蚓体内的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)均有不同程度的上升;暴露28 d后,蚯蚓的基因表达也产生了明显变化.表明金霉素污染对蚯蚓的生长发育具有慢性生态毒性效应,蚯蚓体质量、可溶性蛋白、抗氧化防御酶和基因表达等指标可以作为评价金霉素毒性的生物标志物.Eiseniafoetida was selected to investigate the ecotoxicological effects of chlorotetracycline on the earthworm in soil. The results showed that 1, 10 and 100 mg · kg-1 chlorotetracycline had no significant effects on earthworm＇ s body mass after a 7-d exposure, but it was significantly inhibited by 10, 100 mg · kg-1 chlorotetracycline after 21 days. The soluble protein content of earthworm was induced by 1, 10 and 100 mg · kg-1 chlorotetracycline, and showed a positive response as the concentration increased. Also,the earthworm treated by 1, 10 and 100 mg·kg-1 chlorotetracycline in- duced the increases of SOD, POD and CAT activities to different degrees. The gene expression in earthworm changed significantly after a 28-d exposure. It is suggested that chlorotetracycline had a chronic ecotoxicological effect on earthworm, and the body mass, soluble protein, antioxidant en- zyme and gene expression could be used as the biomarkers to estimate chlorotetracycline toxicity.

关 键 词：金霉素 蚯蚓 土壤污染 抗氧化酶 生态毒性效应 

分 类 号：X53[环境科学与工程—环境工程] X171.5