鼓室内注射地塞米松对豚鼠膜迷路积水及囊性纤维化跨膜转运调节因子表达的影响  

Effects of intratympanic injection of dexamethasone on endolymphatic hydrops and changes in guinea pigs inner ear CFTR expression

作　　者：薛茜[1] 邢巍巍[1] 刘永新[1] 宫亮[1] 

机构地区：[1]辽宁医学院附属第一医院耳鼻咽喉科,辽宁锦州121000

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第19期1483-1486,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：辽宁省教育厅课题资助项目(No:L2010303)

摘　　要：目的:通过观察鼓室注射地塞米松后豚鼠膜迷路积水的变化和囊性纤维化跨膜转运调节因子(CFTR)表达的改变,探讨鼓室内注射糖皮质激素对膜迷路积水的治疗作用及其可能机制。方法:取健康白色红目豚鼠30只,随机分为激素组、积水组、对照组,每组10只。激素组、积水组采用腹腔注射醋酸去氨加压素4μg/kg,连续注射7d后将剂量加大为6μg/kg,再连续注射3d;对照组给予等量生理盐水连续腹腔注射10d。第12天起腹腔常规麻醉后,激素组经鼓室注射地塞米松(5mg/ml,0.5ml),同时积水组、对照组经鼓室给予等量生理盐水(0.5ml),连续注射5d。实验前、后分别行ABR测试听阈值,测试完毕之后取材,行耳蜗苏木精-伊红染色观察膜迷路积水的变化;行免疫组织化学及Western blot检测观察各组耳蜗CFTR的表达情况。结果:实验前各组ABR阈值比较差异无统计学意义(P>0.05),实验后积水组ABR阈值较对照组、激素组明显升高(均P<0.01);激素组较对照组阈值升高(P<0.05)。对照组无膜迷路积水,积水组均出现不同程度膜迷路积水,积水组蜗管与蜗管加前庭阶的面积比值较对照组、激素组明显升高(均P<0.01);激素组较对照组面积比值升高(P<0.05)。CFTR表达主要在血管纹、Corti′s器、螺旋韧带、基膜、耳蜗神经节等处,积水组CFTR表达较对照组、激素组表达增加(均P<0.01);激素组较对照组表达增加(P<0.05)。结论:鼓室注射地塞米松可减轻醋酸去氨加压素引起的豚鼠膜迷路积水,并改善听力;鼓室注射地塞米松可使膜迷路积水豚鼠耳蜗CFTR表达减少,说明鼓室注射地塞米松减轻膜迷路积水的机制可能与CFTR的表达改变有关。Objective：To observe the effect of tympanic injection of dexamethasone in the guinea pig endolymphatic hydrops and the change CFTR expression,to explore the effect of glucocorticoid treatment endolymphatic and its possible mechanism.Method：Thirty guinea pigs were divided into three groups：hormone group,water group,control group.The animals（hormone group,water group）in study were injected DDAVP 4μg/kg in the first 7d,and will increase to 6μg/kg in the second 3d.The control group was given normal saline,continuous10 d.After twelfth days,the hormone group transtympanic injection of dexamethasone（5mg/ml,0.5ml）,and water group,control group transtympanic given normal saline（0.5ml）,continuous injection 5d.Using immunohistochemistry and Western blot to detect the cystic fibrosis transmembrane conductance regulator cochlear factor（CFTR）expression.Result：The water group ABR thresholds was significantly higher than that before the experiment（P〈0.01）,and the water group was significantly higher than the rest of the groups（P〈0.01）;Hormone group compared with the control group increased threshold value（P〈0.05）.The control group had no endolymphatic hydrops,the water group showed varying degrees of endolymphatic hydrops,cochlear duct and vestibular plus cochlear duct area ratio compared with the control group,hormone group was significantly higher（P〈0.01）.hormone group area ratio was higher than the control group（P〈0.05）.CFTR was primarily expressed in the stria vascularis,Corti′s,spiral ligament,basilar membrane,cochlear ganglion,etc.The expression of CFTR in the water group was increased than that in the control group,and the hormone group（P〈0.01）;the expression of hormone group increased compared with the control group（P〈0.05）.Conclusion：Tympanic injection of dexamethasone can alleviate the desmopressin acetatein guinea pigs caused by membranous labyrinth,and the improvement of the hearing;Tympanic injection of dexamethasone can make the endolymphatic h

关 键 词：豚鼠 鼓室 囊性纤维跨膜调节因子 膜迷路积水 地塞米松 

分 类 号：R764[医药卫生—耳鼻咽喉科]