社区获得性耐甲氧西林金黄色葡萄球菌儿童分离株功能基因分型  

Functional gene typing of community-acquired MRSA strains isolated from children

作　　者：程运涛 吴为民 常青 朱健铭 翁幸鐾[4] 欧阳冰 李琴[5] 

机构地区：[1]无锡市第八人民医院检验科,江苏无锡214000 [2]无锡市第八人民医院儿内科,江苏无锡214000 [3]杭州市余杭区中医院检验科,浙江杭州311106 [4]宁波市第一医院检验科,浙江宁波315010 [5]南京医科大学附属无锡市人民医院检验科,江苏无锡214000

出　　处：《中华医院感染学杂志》2014年第20期4967-4969,4995,共4页Chinese Journal of Nosocomiology

基　　金：杭州市科技局科技基金资助项目(20110833B48)

摘　　要：目的调查社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)分离株的毒力基因、耐药基因的分型情况。方法 20株CA-MRSA分离自2010年1-12月儿童专科医院门诊因皮肤软组织感染就诊者,为病灶部拭子样本,采用聚合酶链反应(PCR)的方法对菌株进行了6种真性毒力基因(sasX、pvl、psm-mec、tst、hla、hlg)、4种黏附毒力基因(fnbA、clfA、clfB、icaA)和8种耐药相关基因(mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(4′)、ermA/B/C、tetM、qacA/B、nes)检测,并进行毒力与耐药功能基因分型。结果 20株CA-MRSA对苯唑西林、头孢西丁、亚胺培南的耐药率均为100.0%;3种氨基糖苷类修饰酶基因合并检出18株,阳性率90.0%,20株CA-MRSA毒力与耐药功能基因分型可分13种类型,其中11和19号菌无毒力基因检出率为10.0%。结论对CA-MRSA进行毒力与耐药基因分型尝试国内鲜有报道,MRSA毒力基因的高检出率和菌株致病性高度相关,耐药基因的高检出率和菌株多药耐药表型一致,但未检出sasX与hlg。OBJECTIVE To investigate the genotyping of virulence genes and drug-resistant genes for a group of 20 community-acquired methicillin-resistant Staphylococcus aureus （CA-MRSA） .METHODS Totally 20 strains of MRSA ,which were gained from lesion swab samples from patients suffering from skin or soft tissue infections , were collected from a children′s hospital in one city from Jan .to Dec .2010 .All of them were tested by PCR for six kinds of true virulence genes （sasX ,pvl,psm-mec ,tst,hla,hlg）,four kinds of adhesion virulence genes （fnbA ,clfA ,clfB ,icaA） and eight kinds of drug-resistant genes （mecA ,aac（6′）/aph（2″） ,aph（3′）-Ⅲ ,ant （4′） ,ermA/B/C ,tetM ,qacA/B ,nes） .In addition ,genotyping for virulence and drug resistance was performed . RESULTS The 20 strains of CA-MRSA had 100% resistance to oxacillin ,cefoxitin ,and imipenem .Three kinds of aminoglycoside modifying enzyme genes were found in 18 strains of CA-MRSA and the positive rate was 90 .0% . All the virulence genes and drug-resistant genes of CA-MRSA could be divided into 13 types ,among which the positive rate of nontoxic gene in bacteria No .11 and No .19 was 10 .0% .CONCLUSIONS There are few domestic reports about genotyping of MRSA according to virulence factors and drug-resistance .In this research ,the high positive rate of virulence genes of CA-MRSA was correlated with its pathogenicity ,and the high positive rate of drug-resistant genes was identical with phenotypes of multidrug-resistance .However ,sasX and hlg were tested negative .

关 键 词：社区获得性 耐甲氧西林金黄色葡萄球菌 毒力基因 耐药基因 基因分型 

分 类 号：R378.1[医药卫生—病原生物学]