蒌叶乙醇提取物抗氧化及抗疲劳作用研究  

Antioxidation and antifatigue activities of the ethanolic extract from piper betle

作　　者：纪明慧[1] 郭飞燕[1] 王呈文[1] 陈忠[1,2] 舒火明[1,3] 韩采密 

机构地区：[1]海南师范大学"省部共建-热带药用植物化学"教育部重点实验室,海南海口571158 [2]海南师范大学生命科学院,海南海口571158 [3]海南经贸职业技术学院,海南海口571127

出　　处：《化学研究与应用》2014年第10期1557-1562,共6页Chemical Research and Application

基　　金：海南省自然科学基金项目(213019)资助;海南省大学生科技创新项目(2013116580)资助

摘　　要：以75%乙醇提取蒌叶提取物,运用鲁米诺化学发光体系测定提取物清除羟自由基的能力,并以小白鼠为研究对象,从运动耐力学和生化指标两方面,考察蒌叶乙醇提取物的抗疲劳活性。结果表明:蒌叶乙醇提取物对羟基自由基抑制的IC50为63.65μg·mL-1,其提取物能显著延长小鼠负重游泳时间,明显降低运动后血清尿素氮水平,具有降低小鼠运动后血乳酸曲线下面积的作用。蒌叶乙醇提取物具有抗氧化和缓解体力疲劳的作用,其提取物中抗氧化活性成分有较高的热和光稳定性。The 75%ethanol extract from the leaf of piper betle（ ELPB） was obtained. ELPB was measured the capability for scaven-ging hydroxyl radical by luminol chemiluminescence system. And the anti-fatigue effects was studied in two aspects of exercise per-formance and biochemical indexes by the mice. The results showed that ELPB had good ability to scavenge hydroxyl radicals（ IC50 63. 65mg·mL-1 ）. It also prolonged the swimming time to exhaustion of mice compared to the control. It decreased the levels of blood lactic acid（BLA）and serum urea nitrogen（SUN）. ELPB had marked anti-oxidation effect,and also can alleviate physical fatigue in mice. And the antioxidant component from ELPB had high thermal and light stability.

关 键 词：蒌叶 抗氧化 抗疲劳 流动注射化学发光法 

分 类 号：R285.5[医药卫生—中药学]